TY - JOUR
T1 - Interferon-γ acts directly on rejecting renal allografts to prevent graft necrosis
AU - Halloran, Philip F.
AU - Afrouzian, Marjan
AU - Ramassar, Vido
AU - Urmson, Joan
AU - Zhu, Lin Fu
AU - Helms, Lisa M.H.
AU - Solez, Kim
AU - Kneteman, Norman M.
N1 - Funding Information:
Supported by the Medical Research Council of Canada, Roche Organ Transplant Research Foundation, Kidney Foundation of Canada, Novartis Pharmaceuticals Canada, Inc., Hoffmann-La Roche Canada, Inc., the Muttart Foundation, Royal Canadian Legion.
PY - 2001/1
Y1 - 2001/1
N2 - In transplant rejection interferon (IFN)-γ regulates the recipient immune response but also acts directly on IFN-γ receptors in the graft. We investigated these direct actions by comparing rejecting kidneys from donors lacking IFN-γ receptors (GRKO mice) or control donors (129Sv/J) in CBA recipients. Beginning day 5, 129Sv/J kidneys displayed high major histocompatibility complex (MHC) expression, progressive infiltration by inflammatory cells, but no thrombosis and little necrosis, even at day 21. GRKO kidneys showed increasing fibrin thrombi in small veins, peritubular capillary congestion, hyaline casts, and patchy parenchymal necrosis, progressing to near total necrosis at day 10. Terminal dUTP nick-end labeling assays were positive only in the interstitial infiltrate, confirming that massive cell death in GRKO transplants was not apoptotic. Paradoxically, GRKO kidneys showed little donor MHC induction and less inflammatory infiltration. Both GRKO and 129Sv/J allografts evoked vigorous host immune responses including alloantibody and mRNA for cytotoxic T cell genes (perforin, granzyme B, Fas ligand), anddisplayed similar expression of complement inhibitors (CD46, CD55, CD59). GRKO kidneys displayed less mRNA for inducible nitric oxide synthase and monokine inducible by IFN-γ but increased heme oxygenase-1 mRNA. Thus IFN-γ acting on IFN-γ receptors in allografts promotes infiltration and MHC induction but prevents earlythrombosis, congestion, and necrosis.
AB - In transplant rejection interferon (IFN)-γ regulates the recipient immune response but also acts directly on IFN-γ receptors in the graft. We investigated these direct actions by comparing rejecting kidneys from donors lacking IFN-γ receptors (GRKO mice) or control donors (129Sv/J) in CBA recipients. Beginning day 5, 129Sv/J kidneys displayed high major histocompatibility complex (MHC) expression, progressive infiltration by inflammatory cells, but no thrombosis and little necrosis, even at day 21. GRKO kidneys showed increasing fibrin thrombi in small veins, peritubular capillary congestion, hyaline casts, and patchy parenchymal necrosis, progressing to near total necrosis at day 10. Terminal dUTP nick-end labeling assays were positive only in the interstitial infiltrate, confirming that massive cell death in GRKO transplants was not apoptotic. Paradoxically, GRKO kidneys showed little donor MHC induction and less inflammatory infiltration. Both GRKO and 129Sv/J allografts evoked vigorous host immune responses including alloantibody and mRNA for cytotoxic T cell genes (perforin, granzyme B, Fas ligand), anddisplayed similar expression of complement inhibitors (CD46, CD55, CD59). GRKO kidneys displayed less mRNA for inducible nitric oxide synthase and monokine inducible by IFN-γ but increased heme oxygenase-1 mRNA. Thus IFN-γ acting on IFN-γ receptors in allografts promotes infiltration and MHC induction but prevents earlythrombosis, congestion, and necrosis.
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U2 - 10.1016/S0002-9440(10)63960-0
DO - 10.1016/S0002-9440(10)63960-0
M3 - Article
C2 - 11141495
AN - SCOPUS:0035145029
SN - 0002-9440
VL - 158
SP - 215
EP - 226
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 1
ER -