Interferon action

Effects of mouse α and β interferons on rosette formation, phagocytosis, and surface-antigen expression of cells of the macrophage-type line RAW 309Cr.1

O. Yoshie, I. S. Mellman, R. J. Broeze, Mariano Garcia-Blanco, P. Lengyel

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Treatment with an essentially pure mouse α or β interferon boosts the binding and phagocytosis of opsonized sheep red blood cells by cells of the murine macrophage-like cell line RAW 309Cr.l. The kinetics and the dose dependence of the effects of the two interferons are very similar. The effects depend on continued RNA and protein synthesis, and they diminish after the removal of interferon from the medium. Studies with agents specifically binding FcRI receptors (i.e., IgG2a) and FcRII receptors (i.e., the Fab fragment of the antireceptor monoclonal antibody 2.4G2) revealed a three- to fivefold increase in the level of FcRI receptors per cell and an about twofold increase in that of FcRII receptors per cell after treatment with interferon. The enhanced binding and phagocytosis of opsonized sheep red blood cells by interferon treatment are apparently a consequence of the increased number of Fc receptors. As revealed by studies involving the binding to the cells of labeled monoclonal antibodies to several cell surface antigens, the level of the H-2Dd surface antigen is also selectively increased three- to fourfold in the cells after exposure to interferon.

Original languageEnglish (US)
Pages (from-to)128-140
Number of pages13
JournalCellular Immunology
Volume73
Issue number1
DOIs
StatePublished - 1982
Externally publishedYes

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Rosette Formation
Surface Antigens
Phagocytosis
Interferons
Macrophages
Cell Line
Sheep
Erythrocytes
Monoclonal Antibodies
Immunoglobulin Fab Fragments
Fc Receptors
RNA
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Interferon action : Effects of mouse α and β interferons on rosette formation, phagocytosis, and surface-antigen expression of cells of the macrophage-type line RAW 309Cr.1. / Yoshie, O.; Mellman, I. S.; Broeze, R. J.; Garcia-Blanco, Mariano; Lengyel, P.

In: Cellular Immunology, Vol. 73, No. 1, 1982, p. 128-140.

Research output: Contribution to journalArticle

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