Interferon gamma (IFN-γ) production by gut-associated lymphoid tissue and spleen following oral Salmonella typhimurium challenge

Lakshmi Ramarathinam, Radwan A. Shaban, David Niesel, Gary R. Klimpel

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Although IFN-γ has been shown to play an important role in protection against a systemic S. typhimurium challenge, the in vivo and in vitro production of this cytokine following S. typhimurium infection of the gastrointestinal tract has not been investigated. In this study, IFN-γ production by gut-associated lymphoid tissue and spleen was investigated in mice following oral challenge with S. typhimurium. Cells obtained from the Peyer's patches (PP), mesenteric lymph nodes (MLN) and spleen (Sp) of mice orally challenged with S. typhimurium were assessed for levels of IFN-γ mRNA after varying times following in vivo infection. RNA obtained from the above tissues was subjected to reverse transcription followed by PCR amplification using primers specific for murine IFN-γ. Elevated levels of IFN-γ mRNA were first detected in the PP at 6 h post-challenge. Elevated levels of IFN-γ mRNA were then detected in the MLN at 24 h and in the spleen at 4 days post-challenge. These in vivo results were in agreement with the ability of these lymphoid tissues to produce IFN-γ upon in vitro stimulation with killed S. typhimurium. Neutralization of endogenously produced IFN-γ by administration of mAb to IFN-γ completely abrogated resistance to an oral challenge of S. typhimurium. A significant difference in the percent mortality was observed between the antibody-treated and control groups. Evaluation of bacterial spread in the antibody treated group versus the control group at 4 days following oral challenge revealed higher numbers of bacteria in the spleen and liver of antibody treated mice. These results clearly show that IFN-γ is rapidly produced by gut-associated lymphoid tissue and spleen following oral S. typhimurium infection, and that endogenous production of IFN-γ is essential in host resistance to S. typhimurium.

Original languageEnglish (US)
Pages (from-to)347-356
Number of pages10
JournalMicrobial Pathogenesis
Volume11
Issue number5
DOIs
StatePublished - 1991

Fingerprint

Lymphoid Tissue
Salmonella typhimurium
Interferon-gamma
Spleen
Peyer's Patches
Salmonella Infections
Messenger RNA
Antibodies
Lymph Nodes
Control Groups
Reverse Transcription
Gastrointestinal Tract
RNA
Cytokines
Bacteria
Polymerase Chain Reaction

Keywords

  • gut-associated lymphoid tissue
  • IFN-γ
  • Salmonella typhimurium

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Interferon gamma (IFN-γ) production by gut-associated lymphoid tissue and spleen following oral Salmonella typhimurium challenge. / Ramarathinam, Lakshmi; Shaban, Radwan A.; Niesel, David; Klimpel, Gary R.

In: Microbial Pathogenesis, Vol. 11, No. 5, 1991, p. 347-356.

Research output: Contribution to journalArticle

@article{a15d2758748a4d34bc8a7c754ffeb427,
title = "Interferon gamma (IFN-γ) production by gut-associated lymphoid tissue and spleen following oral Salmonella typhimurium challenge",
abstract = "Although IFN-γ has been shown to play an important role in protection against a systemic S. typhimurium challenge, the in vivo and in vitro production of this cytokine following S. typhimurium infection of the gastrointestinal tract has not been investigated. In this study, IFN-γ production by gut-associated lymphoid tissue and spleen was investigated in mice following oral challenge with S. typhimurium. Cells obtained from the Peyer's patches (PP), mesenteric lymph nodes (MLN) and spleen (Sp) of mice orally challenged with S. typhimurium were assessed for levels of IFN-γ mRNA after varying times following in vivo infection. RNA obtained from the above tissues was subjected to reverse transcription followed by PCR amplification using primers specific for murine IFN-γ. Elevated levels of IFN-γ mRNA were first detected in the PP at 6 h post-challenge. Elevated levels of IFN-γ mRNA were then detected in the MLN at 24 h and in the spleen at 4 days post-challenge. These in vivo results were in agreement with the ability of these lymphoid tissues to produce IFN-γ upon in vitro stimulation with killed S. typhimurium. Neutralization of endogenously produced IFN-γ by administration of mAb to IFN-γ completely abrogated resistance to an oral challenge of S. typhimurium. A significant difference in the percent mortality was observed between the antibody-treated and control groups. Evaluation of bacterial spread in the antibody treated group versus the control group at 4 days following oral challenge revealed higher numbers of bacteria in the spleen and liver of antibody treated mice. These results clearly show that IFN-γ is rapidly produced by gut-associated lymphoid tissue and spleen following oral S. typhimurium infection, and that endogenous production of IFN-γ is essential in host resistance to S. typhimurium.",
keywords = "gut-associated lymphoid tissue, IFN-γ, Salmonella typhimurium",
author = "Lakshmi Ramarathinam and Shaban, {Radwan A.} and David Niesel and Klimpel, {Gary R.}",
year = "1991",
doi = "10.1016/0882-4010(91)90020-B",
language = "English (US)",
volume = "11",
pages = "347--356",
journal = "Microbial Pathogenesis",
issn = "0882-4010",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - Interferon gamma (IFN-γ) production by gut-associated lymphoid tissue and spleen following oral Salmonella typhimurium challenge

AU - Ramarathinam, Lakshmi

AU - Shaban, Radwan A.

AU - Niesel, David

AU - Klimpel, Gary R.

PY - 1991

Y1 - 1991

N2 - Although IFN-γ has been shown to play an important role in protection against a systemic S. typhimurium challenge, the in vivo and in vitro production of this cytokine following S. typhimurium infection of the gastrointestinal tract has not been investigated. In this study, IFN-γ production by gut-associated lymphoid tissue and spleen was investigated in mice following oral challenge with S. typhimurium. Cells obtained from the Peyer's patches (PP), mesenteric lymph nodes (MLN) and spleen (Sp) of mice orally challenged with S. typhimurium were assessed for levels of IFN-γ mRNA after varying times following in vivo infection. RNA obtained from the above tissues was subjected to reverse transcription followed by PCR amplification using primers specific for murine IFN-γ. Elevated levels of IFN-γ mRNA were first detected in the PP at 6 h post-challenge. Elevated levels of IFN-γ mRNA were then detected in the MLN at 24 h and in the spleen at 4 days post-challenge. These in vivo results were in agreement with the ability of these lymphoid tissues to produce IFN-γ upon in vitro stimulation with killed S. typhimurium. Neutralization of endogenously produced IFN-γ by administration of mAb to IFN-γ completely abrogated resistance to an oral challenge of S. typhimurium. A significant difference in the percent mortality was observed between the antibody-treated and control groups. Evaluation of bacterial spread in the antibody treated group versus the control group at 4 days following oral challenge revealed higher numbers of bacteria in the spleen and liver of antibody treated mice. These results clearly show that IFN-γ is rapidly produced by gut-associated lymphoid tissue and spleen following oral S. typhimurium infection, and that endogenous production of IFN-γ is essential in host resistance to S. typhimurium.

AB - Although IFN-γ has been shown to play an important role in protection against a systemic S. typhimurium challenge, the in vivo and in vitro production of this cytokine following S. typhimurium infection of the gastrointestinal tract has not been investigated. In this study, IFN-γ production by gut-associated lymphoid tissue and spleen was investigated in mice following oral challenge with S. typhimurium. Cells obtained from the Peyer's patches (PP), mesenteric lymph nodes (MLN) and spleen (Sp) of mice orally challenged with S. typhimurium were assessed for levels of IFN-γ mRNA after varying times following in vivo infection. RNA obtained from the above tissues was subjected to reverse transcription followed by PCR amplification using primers specific for murine IFN-γ. Elevated levels of IFN-γ mRNA were first detected in the PP at 6 h post-challenge. Elevated levels of IFN-γ mRNA were then detected in the MLN at 24 h and in the spleen at 4 days post-challenge. These in vivo results were in agreement with the ability of these lymphoid tissues to produce IFN-γ upon in vitro stimulation with killed S. typhimurium. Neutralization of endogenously produced IFN-γ by administration of mAb to IFN-γ completely abrogated resistance to an oral challenge of S. typhimurium. A significant difference in the percent mortality was observed between the antibody-treated and control groups. Evaluation of bacterial spread in the antibody treated group versus the control group at 4 days following oral challenge revealed higher numbers of bacteria in the spleen and liver of antibody treated mice. These results clearly show that IFN-γ is rapidly produced by gut-associated lymphoid tissue and spleen following oral S. typhimurium infection, and that endogenous production of IFN-γ is essential in host resistance to S. typhimurium.

KW - gut-associated lymphoid tissue

KW - IFN-γ

KW - Salmonella typhimurium

UR - http://www.scopus.com/inward/record.url?scp=0026252194&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026252194&partnerID=8YFLogxK

U2 - 10.1016/0882-4010(91)90020-B

DO - 10.1016/0882-4010(91)90020-B

M3 - Article

C2 - 1816488

AN - SCOPUS:0026252194

VL - 11

SP - 347

EP - 356

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

SN - 0882-4010

IS - 5

ER -