Interferons as gene activators. Cloning of the 5' terminus and the control segment of an interferon activated gene

H. Samanta, D. A. Engel, H. M. Chao, A. Thakur, Mariano Garcia-Blanco, P. Lengyel

Research output: Contribution to journalArticle

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Abstract

Treatment of cells with interferons induces various mRNAs and the corresponding proteins. We have described previously the isolation of a mouse cDNA clone (cDNA clone 202) which specifies an mRNA whose level is increased 20-fold in beta-interferon-treated Ehrlich ascites tumor cells. The increase is a consequence of an increased rate of transcription. The mRNA encodes a 56,000-dalton protein. We report here the isolation of a genomic clone including the 5' terminus of the 202 gene with the interferon-responsive region. Experiments involving primer extension and protection from cleavage by S1 nuclease revealed the existence of multiple 5' termini of 202 mRNAs in Ehrlich ascites tumor and L(tk-) cells. Treatment with beta-interferon increased the level of these 202 mRNAs with different 5' termini nonuniformly. A 0.8-kilobase DNA segment from the 202 gene (including its 5' flanking region and its 5'-terminal exon) was ligated to the chloramphenicol acetyltransferase gene, and the resulting construct was transfected into mouse L(tk-) cells. Treatment of these cells with beta-interferon increased the expression of the chloramphenicol acetyltransferase gene 5-10-fold. Within the first, untranslated exon of the 202 gene, we found a 29-nucleotide long sequence that is partially homologous to sequences which occur upstream from interferon-inducible human HLA and metallothionein IIA genes (Friedman, R.L., and Stark, G.R. (1985) Nature 314, 637-639).

Original languageEnglish (US)
Pages (from-to)11849-11858
Number of pages10
JournalJournal of Biological Chemistry
Volume261
Issue number25
StatePublished - 1986
Externally publishedYes

Fingerprint

Cloning
Interferons
Organism Cloning
Genes
Interferon-beta
Messenger RNA
Ehrlich Tumor Carcinoma
Chloramphenicol O-Acetyltransferase
Clone Cells
Cells
Tumors
Exons
Complementary DNA
Metallothionein
5' Flanking Region
Transcription
Sequence Homology
Proteins
Therapeutics
Nucleotides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interferons as gene activators. Cloning of the 5' terminus and the control segment of an interferon activated gene. / Samanta, H.; Engel, D. A.; Chao, H. M.; Thakur, A.; Garcia-Blanco, Mariano; Lengyel, P.

In: Journal of Biological Chemistry, Vol. 261, No. 25, 1986, p. 11849-11858.

Research output: Contribution to journalArticle

Samanta, H. ; Engel, D. A. ; Chao, H. M. ; Thakur, A. ; Garcia-Blanco, Mariano ; Lengyel, P. / Interferons as gene activators. Cloning of the 5' terminus and the control segment of an interferon activated gene. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 25. pp. 11849-11858.
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