Interleukin-1β and interferon-γ regulate interleukin-6 production in cultured human intestinal epithelial cells

Alexander A. Parikh, Andrew L. Salzman, Josef E. Fischer, Csaba Szabo, Per Olof Hasselgren

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Recent studies suggest that interleukin-6 (IL-6) is produced in the intestinal mucosa during sepsis and endotoxemia and that the enterocyte may be a source of IL-6 in these conditions. The regulation of IL-6 production in the enterocyte is not fully understood. We tested the hypothesis that IL-6 production in the enterocyte is regulated by proinflammatory cytokines. This was done by treating cultured Caco-2 cells, a transformed human intestinal epithelial cell line, with different concentrations of tumor necrosis factor-α (TNF-α), IL-1β, IL-6 or interferon-γ (IFN-γ). IL-6 production by the Caco-2 cells was determined by ELISA. The expression of IL-6 mRNA was determined by reverse-transcriptase polymerase chain reaction. IL-6 was not produced in unstimulated Caco-2 cells. Treatment of the Caco-2 cells with IL-1/3 resulted in a dose- and time-dependent stimulation of IL-6 production with a maximal effect noted at an IL-1β concentration of .5 ng/mL at 24 h. IFN-γ alone did not stimulate IL-6 production but potentiated the effect of IL-1β in a synergistic fashion. Treatment of the Caco-2 cells with IL-1β induced expression of IL-6 mRNA with a response noticed after 30 min. TNF-α and IL-6 did not influence the production of IL-6 in the Caco-2 cells. The results suggest that enterocyte IL-6 production is stimulated by IL-1β and that this effect is potentiated by IFN-γ. The regulation of IL-6 production in the enterocyte may be specific for IL-1β, since neither TNF nor IL-6 stimulated IL-6 production.

Original languageEnglish (US)
Pages (from-to)249-255
Number of pages7
JournalShock
Volume8
Issue number4
StatePublished - 1997
Externally publishedYes

Fingerprint

Interleukin-1
Interferons
Interleukin-6
Epithelial Cells
Caco-2 Cells
Enterocytes
Tumor Necrosis Factor-alpha
Messenger RNA
Endotoxemia
Interleukin-3
Intestinal Mucosa
Reverse Transcriptase Polymerase Chain Reaction
Cultured Cells

ASJC Scopus subject areas

  • Physiology
  • Critical Care and Intensive Care Medicine

Cite this

Parikh, A. A., Salzman, A. L., Fischer, J. E., Szabo, C., & Hasselgren, P. O. (1997). Interleukin-1β and interferon-γ regulate interleukin-6 production in cultured human intestinal epithelial cells. Shock, 8(4), 249-255.

Interleukin-1β and interferon-γ regulate interleukin-6 production in cultured human intestinal epithelial cells. / Parikh, Alexander A.; Salzman, Andrew L.; Fischer, Josef E.; Szabo, Csaba; Hasselgren, Per Olof.

In: Shock, Vol. 8, No. 4, 1997, p. 249-255.

Research output: Contribution to journalArticle

Parikh, AA, Salzman, AL, Fischer, JE, Szabo, C & Hasselgren, PO 1997, 'Interleukin-1β and interferon-γ regulate interleukin-6 production in cultured human intestinal epithelial cells', Shock, vol. 8, no. 4, pp. 249-255.
Parikh, Alexander A. ; Salzman, Andrew L. ; Fischer, Josef E. ; Szabo, Csaba ; Hasselgren, Per Olof. / Interleukin-1β and interferon-γ regulate interleukin-6 production in cultured human intestinal epithelial cells. In: Shock. 1997 ; Vol. 8, No. 4. pp. 249-255.
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