Interleukin-1 production in tumor cells of human melanoma surgical specimens

Douglas Tyler, G. M. Francis, M. Frederick, A. H. Tran, N. G. Ordonez, J. L. Smith, O. Eton, M. Ross, E. A. Grimm

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

To determine whether IL-1α and/or IL-1β protein is expressed by human melanoma tumor in vivo, we first analyzed nine human melanoma cell lines and optimized the in situ detection of these proteins. Three of the melanoma cell lines stained positively for both IL-1α and IL-1β using immunohistochemistry (IHC). The specificity of IHC was confirmed by the ability of purified recombinant IL-1α and IL-1β protein to abolish the staining after being adsorbed by their respective antibodies before use in IHC. The three positively staining cell lines were also the only lines to demonstrate IL-1 production by western blot analysis as well as IL-1 secretion by ELISA. Next we examined 29 surgically obtained melanoma tumor specimens (6 primary and 23 metastases) that had been formalin fixed and paraffin embedded. Using the same anti-IL-1 antibodies, 5 of 23 metastatic tumors stained positively. None of the 6 primary lesions stained for either IL-1α or IL-1β. Comparison of staining pattern performed on serially sectioned tissue using preimmune serum and antibodies against S-100 protein, melanoma-associated antigen (HMB-45), and CD68 (κP1), which recognizes monocyte-macrophage cell lineage, demonstrates for the first time that IL-1 protein is produced by human melanoma tumor cells in vivo. These findings provide the basis for examination of what may be a previously unrecognized biologically distinct subset of patients.

Original languageEnglish (US)
Pages (from-to)331-340
Number of pages10
JournalJournal of Interferon and Cytokine Research
Volume15
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Interleukin-1
Melanoma
Neoplasms
Immunohistochemistry
Staining and Labeling
Cell Line
Antibodies
Proteins
Melanoma-Specific Antigens
S100 Proteins
Cell Lineage
Paraffin
Formaldehyde
Monocytes
Western Blotting
Enzyme-Linked Immunosorbent Assay
Macrophages
Neoplasm Metastasis

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Virology

Cite this

Tyler, D., Francis, G. M., Frederick, M., Tran, A. H., Ordonez, N. G., Smith, J. L., ... Grimm, E. A. (1995). Interleukin-1 production in tumor cells of human melanoma surgical specimens. Journal of Interferon and Cytokine Research, 15(4), 331-340.

Interleukin-1 production in tumor cells of human melanoma surgical specimens. / Tyler, Douglas; Francis, G. M.; Frederick, M.; Tran, A. H.; Ordonez, N. G.; Smith, J. L.; Eton, O.; Ross, M.; Grimm, E. A.

In: Journal of Interferon and Cytokine Research, Vol. 15, No. 4, 1995, p. 331-340.

Research output: Contribution to journalArticle

Tyler, D, Francis, GM, Frederick, M, Tran, AH, Ordonez, NG, Smith, JL, Eton, O, Ross, M & Grimm, EA 1995, 'Interleukin-1 production in tumor cells of human melanoma surgical specimens', Journal of Interferon and Cytokine Research, vol. 15, no. 4, pp. 331-340.
Tyler D, Francis GM, Frederick M, Tran AH, Ordonez NG, Smith JL et al. Interleukin-1 production in tumor cells of human melanoma surgical specimens. Journal of Interferon and Cytokine Research. 1995;15(4):331-340.
Tyler, Douglas ; Francis, G. M. ; Frederick, M. ; Tran, A. H. ; Ordonez, N. G. ; Smith, J. L. ; Eton, O. ; Ross, M. ; Grimm, E. A. / Interleukin-1 production in tumor cells of human melanoma surgical specimens. In: Journal of Interferon and Cytokine Research. 1995 ; Vol. 15, No. 4. pp. 331-340.
@article{a789e062df4e409d943a9c8f172b0f27,
title = "Interleukin-1 production in tumor cells of human melanoma surgical specimens",
abstract = "To determine whether IL-1α and/or IL-1β protein is expressed by human melanoma tumor in vivo, we first analyzed nine human melanoma cell lines and optimized the in situ detection of these proteins. Three of the melanoma cell lines stained positively for both IL-1α and IL-1β using immunohistochemistry (IHC). The specificity of IHC was confirmed by the ability of purified recombinant IL-1α and IL-1β protein to abolish the staining after being adsorbed by their respective antibodies before use in IHC. The three positively staining cell lines were also the only lines to demonstrate IL-1 production by western blot analysis as well as IL-1 secretion by ELISA. Next we examined 29 surgically obtained melanoma tumor specimens (6 primary and 23 metastases) that had been formalin fixed and paraffin embedded. Using the same anti-IL-1 antibodies, 5 of 23 metastatic tumors stained positively. None of the 6 primary lesions stained for either IL-1α or IL-1β. Comparison of staining pattern performed on serially sectioned tissue using preimmune serum and antibodies against S-100 protein, melanoma-associated antigen (HMB-45), and CD68 (κP1), which recognizes monocyte-macrophage cell lineage, demonstrates for the first time that IL-1 protein is produced by human melanoma tumor cells in vivo. These findings provide the basis for examination of what may be a previously unrecognized biologically distinct subset of patients.",
author = "Douglas Tyler and Francis, {G. M.} and M. Frederick and Tran, {A. H.} and Ordonez, {N. G.} and Smith, {J. L.} and O. Eton and M. Ross and Grimm, {E. A.}",
year = "1995",
language = "English (US)",
volume = "15",
pages = "331--340",
journal = "Journal of Interferon and Cytokine Research",
issn = "1079-9907",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Interleukin-1 production in tumor cells of human melanoma surgical specimens

AU - Tyler, Douglas

AU - Francis, G. M.

AU - Frederick, M.

AU - Tran, A. H.

AU - Ordonez, N. G.

AU - Smith, J. L.

AU - Eton, O.

AU - Ross, M.

AU - Grimm, E. A.

PY - 1995

Y1 - 1995

N2 - To determine whether IL-1α and/or IL-1β protein is expressed by human melanoma tumor in vivo, we first analyzed nine human melanoma cell lines and optimized the in situ detection of these proteins. Three of the melanoma cell lines stained positively for both IL-1α and IL-1β using immunohistochemistry (IHC). The specificity of IHC was confirmed by the ability of purified recombinant IL-1α and IL-1β protein to abolish the staining after being adsorbed by their respective antibodies before use in IHC. The three positively staining cell lines were also the only lines to demonstrate IL-1 production by western blot analysis as well as IL-1 secretion by ELISA. Next we examined 29 surgically obtained melanoma tumor specimens (6 primary and 23 metastases) that had been formalin fixed and paraffin embedded. Using the same anti-IL-1 antibodies, 5 of 23 metastatic tumors stained positively. None of the 6 primary lesions stained for either IL-1α or IL-1β. Comparison of staining pattern performed on serially sectioned tissue using preimmune serum and antibodies against S-100 protein, melanoma-associated antigen (HMB-45), and CD68 (κP1), which recognizes monocyte-macrophage cell lineage, demonstrates for the first time that IL-1 protein is produced by human melanoma tumor cells in vivo. These findings provide the basis for examination of what may be a previously unrecognized biologically distinct subset of patients.

AB - To determine whether IL-1α and/or IL-1β protein is expressed by human melanoma tumor in vivo, we first analyzed nine human melanoma cell lines and optimized the in situ detection of these proteins. Three of the melanoma cell lines stained positively for both IL-1α and IL-1β using immunohistochemistry (IHC). The specificity of IHC was confirmed by the ability of purified recombinant IL-1α and IL-1β protein to abolish the staining after being adsorbed by their respective antibodies before use in IHC. The three positively staining cell lines were also the only lines to demonstrate IL-1 production by western blot analysis as well as IL-1 secretion by ELISA. Next we examined 29 surgically obtained melanoma tumor specimens (6 primary and 23 metastases) that had been formalin fixed and paraffin embedded. Using the same anti-IL-1 antibodies, 5 of 23 metastatic tumors stained positively. None of the 6 primary lesions stained for either IL-1α or IL-1β. Comparison of staining pattern performed on serially sectioned tissue using preimmune serum and antibodies against S-100 protein, melanoma-associated antigen (HMB-45), and CD68 (κP1), which recognizes monocyte-macrophage cell lineage, demonstrates for the first time that IL-1 protein is produced by human melanoma tumor cells in vivo. These findings provide the basis for examination of what may be a previously unrecognized biologically distinct subset of patients.

UR - http://www.scopus.com/inward/record.url?scp=0028956221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028956221&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 331

EP - 340

JO - Journal of Interferon and Cytokine Research

JF - Journal of Interferon and Cytokine Research

SN - 1079-9907

IS - 4

ER -