Interleukin-1 receptor antagonist protein inhibits the synthesis of IgE and proinflammatory cytokines by allergen-stimulated mononuclear cells.

T. C. Sim, K. A. Hilsmeier, L. M. Reece, J. A. Grant, R. Alam

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Abstract

The ability of interleukin-1 (IL-1) to activate diverse cell populations supports its role as a preeminent cytokine in the pathogenesis of chronic inflammation. In this study, we investigated the role of Il-1 and IL-1 receptor antagonist protein (IRAP) in the regulation of allergen-induced synthesis of IgE and proinflammatory cytokines. The temporal expression of IL-1 beta and IRAP during 5-day allergen-activated peripheral mononuclear cell (PMNC) cultures suggested differential production of the two cytokines. To determine the influence of IRAP on IL-1-mediated cellular responses, we cultured PMNC from allergic donors with specific allergens in the presence or absence of IRAP pretreatment. Culture supernatants were assayed for IgE and cytokines using specific enzyme-linked immunosorbent assay. IRAP at concentrations 0.01, 0.1, and 1 microgram/ml decreased the allergen-stimulated IgE synthesis by 33 +/- 7%, 50 +/- 7%, and 66 +/- 5%, respectively (P < 0.05). Increasing the concentration of allergen did not affect the reduction in IgE synthesis observed in the presence of IRAP. Lipopolysaccharide-stimulated IgE synthesis was also significantly inhibited by IRAP (P < 0.05). In parallel experiments, anti-IL-1 beta monoclonal antibody showed a comparable inhibitory pattern on IgE synthesis (P < 0.05). IRAP inhibited the synthesis of interleukin-6, tumor necrosis factor-alpha, and granulocyte/macrophage colony-stimulating factor in a dose-dependent manner (P < 0.05); the mean inhibition was 31 +/- 4%, 75 +/- 5%, and 88 +/- 2%, respectively, at 1 microgram/ml of IRAP.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)473-479
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume11
Issue number4
StatePublished - Oct 1994

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Interleukin 1 Receptor Antagonist Protein
Interleukin-1 Receptors
Allergens
Immunoglobulin E
Cytokines
Proteins
Interleukin-1beta
Interleukin-1
Cells
Immunosorbents
Protein Synthesis Inhibitors
Granulocyte-Macrophage Colony-Stimulating Factor
Cell culture
Lipopolysaccharides
Interleukin-6
Assays
Cell Culture Techniques
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Interleukin-1 receptor antagonist protein inhibits the synthesis of IgE and proinflammatory cytokines by allergen-stimulated mononuclear cells. / Sim, T. C.; Hilsmeier, K. A.; Reece, L. M.; Grant, J. A.; Alam, R.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 11, No. 4, 10.1994, p. 473-479.

Research output: Contribution to journalArticle

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