Interleukin 1 reduces opioid binding in guinea pig brain

Mahmoud Ahmed, J. Q. Llanos, C. A. Dinarello, C. M. Blatteis

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Interleukin 1 (IL1) is a macrophage-derived polypeptide which signals neurons in the preoptic-anterior hypothalamus to initiate fever and the acute-phase glycoprotein response. Recently, increases in cerebrospinal fluid and hypothalamic levels of β-endorphin have been reported during endotoxin (LPS)- and IL1-induced fevers, suggesting that this opioid may participate in the modulation of IL1 effects in the CNS. In this study, we investigated whether purified (human) IL1 influences the specific binding of three prototypic opioid agonists (2-D-alanine-5-L-methionineamide, DAME; (-)-ethylketocyclazocine, EKC; dihydromorphine, DHM) and one antagonist (naloxone) to opioid receptor-enriched membrane preparations in cerebral cortex, hypothalamus, midbrain, pons, medulla, and cerebellum of guinea pig brain. IL1 reduced the binding of these ligands to their receptors during a 30-min incubation. The extent of IL1 inhibition of a given ligand for its binding sites varied according to the brain region; within some regions, the extent of this inhibition also varied with the four ligands tested. But in cortex the effect of IL1 on the specific binding of DHM is dose-dependent. Similar results were obtained with crude homologous IL1. S. enteritidis endotoxin, suspended in pyrogen-free saline at concentrations from 4 to 36 μg/ml, did not inhibit the binding of these opioid ligands to their receptors in any brain region. These results indicate that IL1 interacts with the opiate receptors in guinea pig brain. This interaction, moreover, is not limited to the hypothalamus alone, the primary site of the pyrogenic action of IL1, but also occurs in other brain regions.

Original languageEnglish (US)
Pages (from-to)1149-1154
Number of pages6
JournalPeptides
Volume6
Issue number6
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Interleukin-1
Opioid Analgesics
Brain
Guinea Pigs
Ligands
Opioid Receptors
Endotoxins
Hypothalamus
Dihydromorphine
Fever
Ethylketocyclazocine
Endorphins
Cerebrospinal fluid
Pyrogens
Anterior Hypothalamus
Acute-Phase Reaction
Pons
Acute-Phase Proteins
Macrophages
Naloxone

Keywords

  • Brain regions
  • Endotoxin
  • Fever
  • Hypothalamus
  • Naloxone
  • Opioid agonists
  • Receptors

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Ahmed, M., Llanos, J. Q., Dinarello, C. A., & Blatteis, C. M. (1985). Interleukin 1 reduces opioid binding in guinea pig brain. Peptides, 6(6), 1149-1154. https://doi.org/10.1016/0196-9781(85)90442-5

Interleukin 1 reduces opioid binding in guinea pig brain. / Ahmed, Mahmoud; Llanos, J. Q.; Dinarello, C. A.; Blatteis, C. M.

In: Peptides, Vol. 6, No. 6, 1985, p. 1149-1154.

Research output: Contribution to journalArticle

Ahmed, M, Llanos, JQ, Dinarello, CA & Blatteis, CM 1985, 'Interleukin 1 reduces opioid binding in guinea pig brain', Peptides, vol. 6, no. 6, pp. 1149-1154. https://doi.org/10.1016/0196-9781(85)90442-5
Ahmed, Mahmoud ; Llanos, J. Q. ; Dinarello, C. A. ; Blatteis, C. M. / Interleukin 1 reduces opioid binding in guinea pig brain. In: Peptides. 1985 ; Vol. 6, No. 6. pp. 1149-1154.
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