Interleukin-10 in human milk

Roberto Garofalo, S. Chheda, F. Mei, K. H. Palkowetz, H. E. Rudloff, F. C. Schmalstieg, D. K. Rassin, A. S. Goldman

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The concentrations of immunoreactive IL-10 in the aqueous fraction of 20 specimens of human milk obtained during the first 80 h of lactation and stored at -60°C ranged from 66 to 9301 pg/mL (mean ± SD, 3304 ± 3127 pg/mL). IL-10 was present also in the lipid layer of milk. Gel filtration revealed that IL-10 was located in a high molecular weight fraction, where certain other cytokines in human milk have been found. In addition, immunoreactive IL-10 in milk increased after treatment with sodium taurocholate. Bioactive IL-10 was demonstrated by the finding that human milk inhibited [3H]thymidine uptake by human blood lymphocytes and that inhibition was partly over come by concomitant incubation with antibodies to human IL-10, IL-10 mRNA but no protein product was found in cultured human mammary epithelial cells. Some IL-10 was associated with preparations of human milk leukocytes, but the data did not suggest that the cells were producing the cytokine. Bioactive IL-10 in a possible protected compartment suggests that IL-10 in human milk may have immunomodulating, antiinflammatory effects on the alimentary tract of the recipient infant.

Original languageEnglish (US)
Pages (from-to)444-449
Number of pages6
JournalPediatric Research
Issue number4 I
StatePublished - 1995


ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Garofalo, R., Chheda, S., Mei, F., Palkowetz, K. H., Rudloff, H. E., Schmalstieg, F. C., Rassin, D. K., & Goldman, A. S. (1995). Interleukin-10 in human milk. Pediatric Research, 37(4 I), 444-449.