Interleukin-10 in human milk

Roberto Garofalo, S. Chheda, F. Mei, K. H. Palkowetz, H. E. Rudloff, F. C. Schmalstieg, D. K. Rassin, A. S. Goldman

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Abstract

The concentrations of immunoreactive IL-10 in the aqueous fraction of 20 specimens of human milk obtained during the first 80 h of lactation and stored at -60°C ranged from 66 to 9301 pg/mL (mean ± SD, 3304 ± 3127 pg/mL). IL-10 was present also in the lipid layer of milk. Gel filtration revealed that IL-10 was located in a high molecular weight fraction, where certain other cytokines in human milk have been found. In addition, immunoreactive IL-10 in milk increased after treatment with sodium taurocholate. Bioactive IL-10 was demonstrated by the finding that human milk inhibited [3H]thymidine uptake by human blood lymphocytes and that inhibition was partly over come by concomitant incubation with antibodies to human IL-10, IL-10 mRNA but no protein product was found in cultured human mammary epithelial cells. Some IL-10 was associated with preparations of human milk leukocytes, but the data did not suggest that the cells were producing the cytokine. Bioactive IL-10 in a possible protected compartment suggests that IL-10 in human milk may have immunomodulating, antiinflammatory effects on the alimentary tract of the recipient infant.

Original languageEnglish (US)
Pages (from-to)444-449
Number of pages6
JournalPediatric Research
Volume37
Issue number4 I
StatePublished - 1995

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Human Milk
Interleukin-10
Milk
Cytokines
Taurocholic Acid
Lactation
Thymidine
Gel Chromatography
Breast
Leukocytes
Anti-Inflammatory Agents
Molecular Weight
Epithelial Cells
Lymphocytes
Lipids
Messenger RNA
Antibodies

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Garofalo, R., Chheda, S., Mei, F., Palkowetz, K. H., Rudloff, H. E., Schmalstieg, F. C., ... Goldman, A. S. (1995). Interleukin-10 in human milk. Pediatric Research, 37(4 I), 444-449.

Interleukin-10 in human milk. / Garofalo, Roberto; Chheda, S.; Mei, F.; Palkowetz, K. H.; Rudloff, H. E.; Schmalstieg, F. C.; Rassin, D. K.; Goldman, A. S.

In: Pediatric Research, Vol. 37, No. 4 I, 1995, p. 444-449.

Research output: Contribution to journalArticle

Garofalo, R, Chheda, S, Mei, F, Palkowetz, KH, Rudloff, HE, Schmalstieg, FC, Rassin, DK & Goldman, AS 1995, 'Interleukin-10 in human milk', Pediatric Research, vol. 37, no. 4 I, pp. 444-449.
Garofalo R, Chheda S, Mei F, Palkowetz KH, Rudloff HE, Schmalstieg FC et al. Interleukin-10 in human milk. Pediatric Research. 1995;37(4 I):444-449.
Garofalo, Roberto ; Chheda, S. ; Mei, F. ; Palkowetz, K. H. ; Rudloff, H. E. ; Schmalstieg, F. C. ; Rassin, D. K. ; Goldman, A. S. / Interleukin-10 in human milk. In: Pediatric Research. 1995 ; Vol. 37, No. 4 I. pp. 444-449.
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AU - Chheda, S.

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AU - Palkowetz, K. H.

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AU - Schmalstieg, F. C.

AU - Rassin, D. K.

AU - Goldman, A. S.

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