Interleukin-18 primes human basophilic KU812 cells for higher leukotriene synthesis

Masafumi Zaitsu, Fumio Yamasaki, Eiichi Ishii, Terumi Midoro-Horiuti, Randall M. Goldblum, Yuhei Hamasaki

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The human basophilic cell line KU812 that is an established tool for studying the function of human basophils, is differentiated into mature basophils by interleukin (IL-3) or other agents. However, whether leukotrienes (LTs)-synthesis is affected by cytokines in KU812 cells remains unknown. KU812 cells were incubated with IL-3, IL-4, IL-6, IL-13 or IL-18 for up to 14 days. The A23187 stimulated- and IgE cross-linked-synthesis of LTC 4 and LTB 4 were measured using an enzyme immunoassay (EIA). The expression of messenger RNA (mRNA) for LT-synthesizing enzymes was examined by reverse transcriptase polymerase chain reaction (RT-PCR), and the expression of 5-lipoxygenase (5-LO) was examined by immunostaining. Incubation with IL-3 (10 ng/ml) and IL-18 (10 ng/ml) induced the expression of 5-LO. A23187stimulated LT-synthesis and IgE cross-linked LT-synthesis were enhanced after incubation with IL-3 or IL-18. These results indicated that IL-3 and IL-18 primed human basophils for higher LT-synthesis. Thus, both IL-3 and IL-18 might be important factors for regulating LT-synthesis during the differentiation of human basophils.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume74
Issue number1
DOIs
StatePublished - Jan 1 2006

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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