Interleukin-8, aquaporin-1, and inducible nitric oxide synthase in smoke and burn injured sheep treated with percutaneous carbon dioxide removal

Frank C. Schmalstieg, John Chow, Clare Savage, Helen E. Rudloff, Kimberly H. Palkowetz, Joseph B. Zwischenberger

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We previously showed that a percutaneous arteriovenous gas exchanger was effective in removing CO2 and reversing respiratory failure in an ovine model of adult respiratory distress syndrome (ARDS) produced by smoke inhalation and burn injury (Alpard et al., Ann Surg 230:215-224, 1999). In this study, we tested the hypothesis that arteriovenous CO2 removal (AVCO2R) lessened endogenous inflammation in the lung. Myeloperoxidase activity, aquaporin-1 (AQP-1), interleukin-8 (IL-8), and inducible nitric oxide synthase mRNAs as well as aquaporin-1, and IL-8 protein were measured in ovine lung tissue. Lung tissue was taken at 96 h (time of sacrifice) from animals with combined smoke inhalation and 40% third degree dermal burn and subsequently treated with AVCO2R or sham (ventilator alone) after onset of ARDS (PaO2:FiO2 ratio of < 200). Myeloperoxidase activity was 1.862 ± 0.302 U/mg protein in the ventilator group and 0.830 ± 0.141 in the AVCO2R plus ventilator group. AQP-1 mRNA was 140,482 ± 31,702 copies/μg total RNA in the ventilator group and 61,854 ± 22,433 copies/μg total RNA in the AVCO2R plus ventilator group (p = 0.076). mRNA for IL-8 mRNA in the ventilator alone treated animals was 74,000 ± 3,300 copies/μg total RNA compared to < 1,000 copies/μg total RNA in the ventilator plus AVCO2R group. This result was highly significant (p < 0.001) Inducible nitric oxide synthase mRNA was 7,853 ± 2,229 copies/μg total RNA for the AVCO2R group and 5,854 ± 2,070 copies/μg total RNA for the ventilator managed animals. These differences were not statistically significant (p = 0.54). Percutaneous AVCO2R produced a specific decrease in IL-8 in the smoke and burn injured animals. Furthermore, this effect was consistent with cell signaling mechanisms that increase the expression of IL-8 by cyclic stretching and the observed reduction in the number of neutrophils in the lung parenchyma. Therefore, we speculate that the mechanism by which CO2 removal exerts a beneficial effect may be due to both decreases in ventilatory requirements, with an accompanying reduction in alveolar stretching, and reduction of neutrophil numbers in lung tissue.

Original languageEnglish (US)
Pages (from-to)365-371
Number of pages7
JournalASAIO Journal
Volume47
Issue number4
StatePublished - 2001

Fingerprint

Aquaporin 1
Nitric oxide
Nitric Oxide Synthase Type II
Mechanical Ventilators
Interleukin-8
Smoke
Carbon Dioxide
Sheep
Carbon dioxide
RNA
Animals
Messenger RNA
Lung
Adult Respiratory Distress Syndrome
Tissue
Peroxidase
Stretching
Inhalation Burns
Neutrophils
Smoke Inhalation Injury

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Schmalstieg, F. C., Chow, J., Savage, C., Rudloff, H. E., Palkowetz, K. H., & Zwischenberger, J. B. (2001). Interleukin-8, aquaporin-1, and inducible nitric oxide synthase in smoke and burn injured sheep treated with percutaneous carbon dioxide removal. ASAIO Journal, 47(4), 365-371.

Interleukin-8, aquaporin-1, and inducible nitric oxide synthase in smoke and burn injured sheep treated with percutaneous carbon dioxide removal. / Schmalstieg, Frank C.; Chow, John; Savage, Clare; Rudloff, Helen E.; Palkowetz, Kimberly H.; Zwischenberger, Joseph B.

In: ASAIO Journal, Vol. 47, No. 4, 2001, p. 365-371.

Research output: Contribution to journalArticle

Schmalstieg, FC, Chow, J, Savage, C, Rudloff, HE, Palkowetz, KH & Zwischenberger, JB 2001, 'Interleukin-8, aquaporin-1, and inducible nitric oxide synthase in smoke and burn injured sheep treated with percutaneous carbon dioxide removal', ASAIO Journal, vol. 47, no. 4, pp. 365-371.
Schmalstieg, Frank C. ; Chow, John ; Savage, Clare ; Rudloff, Helen E. ; Palkowetz, Kimberly H. ; Zwischenberger, Joseph B. / Interleukin-8, aquaporin-1, and inducible nitric oxide synthase in smoke and burn injured sheep treated with percutaneous carbon dioxide removal. In: ASAIO Journal. 2001 ; Vol. 47, No. 4. pp. 365-371.
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abstract = "We previously showed that a percutaneous arteriovenous gas exchanger was effective in removing CO2 and reversing respiratory failure in an ovine model of adult respiratory distress syndrome (ARDS) produced by smoke inhalation and burn injury (Alpard et al., Ann Surg 230:215-224, 1999). In this study, we tested the hypothesis that arteriovenous CO2 removal (AVCO2R) lessened endogenous inflammation in the lung. Myeloperoxidase activity, aquaporin-1 (AQP-1), interleukin-8 (IL-8), and inducible nitric oxide synthase mRNAs as well as aquaporin-1, and IL-8 protein were measured in ovine lung tissue. Lung tissue was taken at 96 h (time of sacrifice) from animals with combined smoke inhalation and 40{\%} third degree dermal burn and subsequently treated with AVCO2R or sham (ventilator alone) after onset of ARDS (PaO2:FiO2 ratio of < 200). Myeloperoxidase activity was 1.862 ± 0.302 U/mg protein in the ventilator group and 0.830 ± 0.141 in the AVCO2R plus ventilator group. AQP-1 mRNA was 140,482 ± 31,702 copies/μg total RNA in the ventilator group and 61,854 ± 22,433 copies/μg total RNA in the AVCO2R plus ventilator group (p = 0.076). mRNA for IL-8 mRNA in the ventilator alone treated animals was 74,000 ± 3,300 copies/μg total RNA compared to < 1,000 copies/μg total RNA in the ventilator plus AVCO2R group. This result was highly significant (p < 0.001) Inducible nitric oxide synthase mRNA was 7,853 ± 2,229 copies/μg total RNA for the AVCO2R group and 5,854 ± 2,070 copies/μg total RNA for the ventilator managed animals. These differences were not statistically significant (p = 0.54). Percutaneous AVCO2R produced a specific decrease in IL-8 in the smoke and burn injured animals. Furthermore, this effect was consistent with cell signaling mechanisms that increase the expression of IL-8 by cyclic stretching and the observed reduction in the number of neutrophils in the lung parenchyma. Therefore, we speculate that the mechanism by which CO2 removal exerts a beneficial effect may be due to both decreases in ventilatory requirements, with an accompanying reduction in alveolar stretching, and reduction of neutrophil numbers in lung tissue.",
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