Interleukin (IL)-1β in tracheal aspirates from premature infants induces airway epithelial cell IL-8 expression via an NF-κB dependent pathway

Thomas K. Shimotake, Farzana M. Izhar, Kandelaria Rumilla, Jing Li, Alan Tan, Kristen Page, Allan R. Brasier, Michael D. Schreiber, Marc B. Hershenson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Tracheal aspirate IL-8 concentration and airway epithelial cell IL-8 expression are each increased in premature infants undergoing mechanical ventilation. We sought to determine the cytokines responsible for IL-8 expression in this context. Tracheal aspirates were collected from 18 mechanically ventilated premature infants. IL-8 protein abundance was high in tracheal aspirates from ventilated premature infants (mean, 5806 ± 4923 pg/mL). IL-1α (mean, 20 ± 6 pg/mL), IL-1β (mean 67 ± 46 pg/mL), and tumor necrosis factor (TNF)-α (mean, 8 ± 2 pg/mL) were also found. Incubation of tracheal aspirates with 16HBE14o- human bronchial epithelial cells increased IL-8 protein in both cell lysates and supernatants, as well as transcription from the IL-8 promoter. Aspirates also induced nuclear factor (NF)-κB activation. Mutation of the IL-8 promoter NF-κB site abolished aspirate-induced IL-8 transcription. Endotoxin concentrations in the tracheal aspirates were negligible and incapable of inducing IL-8 promoter activity. Finally, incubation of tracheal aspirates with a neutralizing antibody against IL-1β reduced epithelial cell IL-8 production, whereas neutralizing antibodies against IL-1α and TNF-α had no effect. We conclude that airway fluid from mechanically ventilated premature infants contains soluble factors capable of inducing airway epithelial cell IL-8 expression via a NF-κB-dependent pathway, and that IL-1β plays a specific role in this process.

Original languageEnglish (US)
Pages (from-to)907-913
Number of pages7
JournalPediatric Research
Volume56
Issue number6
DOIs
StatePublished - Dec 2004

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Interleukin-8
Interleukin-1
Premature Infants
Epithelial Cells
Neutralizing Antibodies
Tumor Necrosis Factor-alpha
Artificial Respiration
Endotoxins
Proteins
Cytokines
Mutation

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Interleukin (IL)-1β in tracheal aspirates from premature infants induces airway epithelial cell IL-8 expression via an NF-κB dependent pathway. / Shimotake, Thomas K.; Izhar, Farzana M.; Rumilla, Kandelaria; Li, Jing; Tan, Alan; Page, Kristen; Brasier, Allan R.; Schreiber, Michael D.; Hershenson, Marc B.

In: Pediatric Research, Vol. 56, No. 6, 12.2004, p. 907-913.

Research output: Contribution to journalArticle

Shimotake, TK, Izhar, FM, Rumilla, K, Li, J, Tan, A, Page, K, Brasier, AR, Schreiber, MD & Hershenson, MB 2004, 'Interleukin (IL)-1β in tracheal aspirates from premature infants induces airway epithelial cell IL-8 expression via an NF-κB dependent pathway', Pediatric Research, vol. 56, no. 6, pp. 907-913. https://doi.org/10.1203/01.PDR.0000145274.47221.10
Shimotake, Thomas K. ; Izhar, Farzana M. ; Rumilla, Kandelaria ; Li, Jing ; Tan, Alan ; Page, Kristen ; Brasier, Allan R. ; Schreiber, Michael D. ; Hershenson, Marc B. / Interleukin (IL)-1β in tracheal aspirates from premature infants induces airway epithelial cell IL-8 expression via an NF-κB dependent pathway. In: Pediatric Research. 2004 ; Vol. 56, No. 6. pp. 907-913.
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