Intermolecular contacts within sickle hemoglobin fibers

Stanley Watowich, Leon J. Gross, Robert Josephs

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have combined x-ray crystallographic coordinates of sickle hemoglobin (HbS) molecules with electron microscopic 3-D reconstruction data of HbS fibers to calculate residues involved in intermolecular contacts within the clinically relevant HbS fiber. The model accounts for the action of 80% of the fifty five point mutations of HbS whose effect on fiber formation has been studied. For instance, fiber formation is affected by thirty-three mutants. Of these mutants, thirty-one occur at the calculated intermolecular contact sites. Fiber formation is not affected by 28 known HbS mutants; 16 of these mutations occur at residues not involved in intermolecular contacts. The 12 mutations that occur at contact sites are postulated to not significantly increase fiber formation energy. The number of intermolecular contacts decreases with radius until, at the periphery of the particle there are no contacts. We suggest that this observation accounts for the limited radial growth of the particle and that a similar mechanism may be a factor in limiting the size of other helical particles.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsRudolph P. Guzik, Hans E. Eppinger, Richard E. Gillespie, Mary K. Dubiel, James E. Pearson
PublisherPubl by Int Soc for Optical Engineering
Pages316-323
Number of pages8
Volume1396
StatePublished - 1991
Externally publishedYes
EventApplications of Optical Engineering: Proceedings of OE/Midwest '90 - Rosemont, IL, USA
Duration: Sep 27 1990Sep 28 1990

Other

OtherApplications of Optical Engineering: Proceedings of OE/Midwest '90
CityRosemont, IL, USA
Period9/27/909/28/90

Fingerprint

Hemoglobin
hemoglobin
fibers
Fibers
mutations
energy of formation
X rays
Molecules
radii
Electrons
molecules
electrons
x rays

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Watowich, S., Gross, L. J., & Josephs, R. (1991). Intermolecular contacts within sickle hemoglobin fibers. In R. P. Guzik, H. E. Eppinger, R. E. Gillespie, M. K. Dubiel, & J. E. Pearson (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1396, pp. 316-323). Publ by Int Soc for Optical Engineering.

Intermolecular contacts within sickle hemoglobin fibers. / Watowich, Stanley; Gross, Leon J.; Josephs, Robert.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Rudolph P. Guzik; Hans E. Eppinger; Richard E. Gillespie; Mary K. Dubiel; James E. Pearson. Vol. 1396 Publ by Int Soc for Optical Engineering, 1991. p. 316-323.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Watowich, S, Gross, LJ & Josephs, R 1991, Intermolecular contacts within sickle hemoglobin fibers. in RP Guzik, HE Eppinger, RE Gillespie, MK Dubiel & JE Pearson (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 1396, Publ by Int Soc for Optical Engineering, pp. 316-323, Applications of Optical Engineering: Proceedings of OE/Midwest '90, Rosemont, IL, USA, 9/27/90.
Watowich S, Gross LJ, Josephs R. Intermolecular contacts within sickle hemoglobin fibers. In Guzik RP, Eppinger HE, Gillespie RE, Dubiel MK, Pearson JE, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1396. Publ by Int Soc for Optical Engineering. 1991. p. 316-323
Watowich, Stanley ; Gross, Leon J. ; Josephs, Robert. / Intermolecular contacts within sickle hemoglobin fibers. Proceedings of SPIE - The International Society for Optical Engineering. editor / Rudolph P. Guzik ; Hans E. Eppinger ; Richard E. Gillespie ; Mary K. Dubiel ; James E. Pearson. Vol. 1396 Publ by Int Soc for Optical Engineering, 1991. pp. 316-323
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