Interspecific hybridization and genetic variability of Phlebotomus sandflies

K. N. Ghosh, J. M. Mukhopadhyay, H. Guzman, R. B. Tesh, L. E. Munstermann

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The first successful hybridization is reported between Phlebotomus papatasi and P. duboscqi, two important Old World sandfly vectors of leishmaniasis and other diseases. Laboratory strains of P. papatasi and P. duboscqi were separable by six diagnostic enzyme loci: Est-3, Idh-1, Mdh-2, Mpi, Tre-1 and Tre-3. Hybrids between the two species were verified by the recovery of heterozygous isozyme patterns for the diagnostic loci. No F2 or backcross progeny were obtained. P. papatasi was separated from P. bergeroti by three diagnostic enzyme loci: Est-3, Mpi and Pgd. The isozyme patterns of P. bergeroti contain elements of both P. duboscqi and P. papatasi, although seven diagnostic loci (Est-3, Idh-1, Me, Mpi, Pgd, Tre-1 and Tre-3) separated P. bergeroti from P. duboscqi. Genetic variability profiles of the three species were established for 20 enzyme loci. Three geographically distant strains of P. papatasi from Calcutta, Maharashtra and Israel had isozyme genetic distances of < 0.05. The recently established Calcutta strain showed an unexpectedly low genetic variability with only one (Idh-2) of 20 loci being polymorphic (average heterozygosity of 1.9%) in contrast to 5-8 polymorphic loci (10-12% heterozygosity) in the Maharashtra and Israel strains. Mass and single pair crosses between the three P. papatasi strains were fertile with normal progeny numbers. Thus we found no signs of speciation in P. papatasi.

Original languageEnglish (US)
Pages (from-to)78-88
Number of pages11
JournalMedical and Veterinary Entomology
Volume13
Issue number1
DOIs
StatePublished - Feb 1999

Fingerprint

Phlebotomus
Phlebotomus papatasi
Genetic Hybridization
Psychodidae
Phlebotominae
interspecific hybridization
Isoenzymes
Israel
enzyme
heterozygosity
genetic variation
loci
Enzymes
leishmaniasis
Phlebotomus duboscqi
Leishmaniasis
isozymes
enzymes
India
transposons

Keywords

  • Hybrid sterility
  • India
  • Isozyme electrophoresis
  • Israel
  • Leishmaniasis vectors
  • P. duboscqi
  • P. papatasi
  • Phlebotomus bergeroti
  • Population genetics
  • Sand flies

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Ghosh, K. N., Mukhopadhyay, J. M., Guzman, H., Tesh, R. B., & Munstermann, L. E. (1999). Interspecific hybridization and genetic variability of Phlebotomus sandflies. Medical and Veterinary Entomology, 13(1), 78-88. https://doi.org/10.1046/j.1365-2915.1999.00140.x

Interspecific hybridization and genetic variability of Phlebotomus sandflies. / Ghosh, K. N.; Mukhopadhyay, J. M.; Guzman, H.; Tesh, R. B.; Munstermann, L. E.

In: Medical and Veterinary Entomology, Vol. 13, No. 1, 02.1999, p. 78-88.

Research output: Contribution to journalArticle

Ghosh, KN, Mukhopadhyay, JM, Guzman, H, Tesh, RB & Munstermann, LE 1999, 'Interspecific hybridization and genetic variability of Phlebotomus sandflies', Medical and Veterinary Entomology, vol. 13, no. 1, pp. 78-88. https://doi.org/10.1046/j.1365-2915.1999.00140.x
Ghosh, K. N. ; Mukhopadhyay, J. M. ; Guzman, H. ; Tesh, R. B. ; Munstermann, L. E. / Interspecific hybridization and genetic variability of Phlebotomus sandflies. In: Medical and Veterinary Entomology. 1999 ; Vol. 13, No. 1. pp. 78-88.
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