Intersubunit communications in Escherichia coli cyclic AMP receptor protein

Studies of the ligand binding domain

Ewa Heyduk, Tomasz Heyduk, James Lee

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Escherichia coli cAMP receptor protein (CRP) is a homodimer in which each subunit is composed of two domains. The C-terminal domain is responsible for DNA recognition, whereas the larger N-terminal domain is involved in cAMP binding. Biochemical and genetic evidence suggests that both intersubunit and interdomain interactions play important roles in the regulatory mechanism of this protein. Essentially all intersubunit contacts occur via a long C-helix which is a part of the N-terminal domain. In this work, intersubunit interactions in CRP were studied with the use of two proteolytic fragments of the protein. Subtilisin digestion produces a fragment (S-CRP) which includes residues 1-117 and in which about 85% of the C-helix is removed, whereas chymotrypsin digestion produces a fragment (CH-CRP) consisting of residues 1-136, in which the whole C-helix is preserved. Both fragments were purified and subjected to functional tests which included cAMP binding, subunit assembly, and hydrodynamic properties in the presence and absence of cAMP. S-CRP binds cAMP with a similar affinity to that of native CRP but with reduced cooperativity. CH-CRP exhibits about 1 order of magnitude tighter binding of cAMP than S-CRP or CRP and the highest degree of negative cooperativity. Both fragments are dimeric with dimerization constants around 108 M-1. Ligand binding promotes dimerization and induces a small contraction of both S-CRP and CH-CRP. There is no apparent correlation between dimer stability and cooperativity of ligand binding. Although residues 117-136 in the C-helix are apparently not critical for dimer stability, they may be important for intersubunit communication that leads to cooperativity in ligand binding.

Original languageEnglish (US)
Pages (from-to)3682-3688
Number of pages7
JournalBiochemistry®
Volume31
Issue number14
StatePublished - 1992

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Cyclic AMP Receptor Protein
Escherichia coli Proteins
Escherichia coli
Ligands
Communication
Dimerization
Dimers
Digestion
Subtilisin
Chymotrypsin
Hydrodynamics
Molecular Biology
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Intersubunit communications in Escherichia coli cyclic AMP receptor protein : Studies of the ligand binding domain. / Heyduk, Ewa; Heyduk, Tomasz; Lee, James.

In: Biochemistry®, Vol. 31, No. 14, 1992, p. 3682-3688.

Research output: Contribution to journalArticle

Heyduk, Ewa ; Heyduk, Tomasz ; Lee, James. / Intersubunit communications in Escherichia coli cyclic AMP receptor protein : Studies of the ligand binding domain. In: Biochemistry®. 1992 ; Vol. 31, No. 14. pp. 3682-3688.
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