Intestinal glutamine metabolism after massive small bowel resection

Vicki Klimberg, Wiley W. Souba, Rabih M. Salloum, Daniel T. Holley, R. Dean Hautamaki, David J. Dolson, Edward M. Copeland

Research output: Contribution to journalArticle

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Abstract

Gut glutamine utilization after massive small bowel resection was studied to gain further insight into the alterations and adaptations in intestinal glutamine metabolism that occur during the development of post-resectional hyperplasia. After resection of the middle 60% of the small intestine in the rat, gut glutamine metabolism was studied immediately and 1, 2, and 3 weeks later. Whole gut glutamine extraction was 22% in sham controls and it acutely declined to 12% (p<0.01) after bowel resection. Extraction increased to 31% 1 week later (p<0.05) and then returned to normal by week 2. Gut ammonia release decreased after massive small bowel resection, whereas intestinal alanine release increased. The increase in gut glutamine extraction at 1 week occurred at a time when jejunal and ileal DNA and protein content were markedly increased (p<0.01). Intestinal glutaminase content declined initially and then increased by the third week after bowel resection (p<0.01). With time, increases in gut cellularity and glutaminase content are associated with gut glutamine utilization in the shortened small bowel that is equal to that of the intact unresected intestine.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalThe American Journal of Surgery
Volume159
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

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Glutamine
Glutaminase
Gastrointestinal Contents
Ammonia
Alanine
Small Intestine
Intestines
Hyperplasia
DNA
Proteins

ASJC Scopus subject areas

  • Surgery

Cite this

Klimberg, V., Souba, W. W., Salloum, R. M., Holley, D. T., Hautamaki, R. D., Dolson, D. J., & Copeland, E. M. (1990). Intestinal glutamine metabolism after massive small bowel resection. The American Journal of Surgery, 159(1), 27-33. https://doi.org/10.1016/S0002-9610(05)80603-8

Intestinal glutamine metabolism after massive small bowel resection. / Klimberg, Vicki; Souba, Wiley W.; Salloum, Rabih M.; Holley, Daniel T.; Hautamaki, R. Dean; Dolson, David J.; Copeland, Edward M.

In: The American Journal of Surgery, Vol. 159, No. 1, 1990, p. 27-33.

Research output: Contribution to journalArticle

Klimberg, V, Souba, WW, Salloum, RM, Holley, DT, Hautamaki, RD, Dolson, DJ & Copeland, EM 1990, 'Intestinal glutamine metabolism after massive small bowel resection', The American Journal of Surgery, vol. 159, no. 1, pp. 27-33. https://doi.org/10.1016/S0002-9610(05)80603-8
Klimberg, Vicki ; Souba, Wiley W. ; Salloum, Rabih M. ; Holley, Daniel T. ; Hautamaki, R. Dean ; Dolson, David J. ; Copeland, Edward M. / Intestinal glutamine metabolism after massive small bowel resection. In: The American Journal of Surgery. 1990 ; Vol. 159, No. 1. pp. 27-33.
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