Intestinal microvascular patterns during hemorrhagic shock

Sergio Morini, Wael Yacoub, Cristiana Rastellini, Eugenio Gaudio, Simon C. Watkins, Luca Cicalese

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

While injuries due to a hypoxic state commonly appear later in both intestinal crypts and basal portion of the villi than in the apical portion, a nonhomogeneous distribution of blood flow in the intestinal mucosa may be supposed. The presence of two different microvascular plexuses inside the mucosa, corresponding to the cryptal plexus and the villous plexus, supports the above hypothesis. This work studies the intestinal microvasculature in shocked versus normal rats. Forty-five rats were divided into four groups to study the histological damage and the microvascular bed by ink injection, fluorescent microsphere infusion, and resin injection for scanning electron microscopy (SEM) of vascular corrosion cast (VCC) observations. An infusion pressure of 100 ± 5 mm Hg was used in control animals, while 30 ± 5 mm Hg infusion pressure was adopted for controls as well as for shocked animals to simulate physiological or shock conditions. Hemorrhagic shock was induced by removing blood and maintaining a mean arterial pressure of 30 ± 5 mm Hg for 45-120 mins. A close connection among the patterns of microvasculature obtained with VCC and ink injection technique can be appreciated. In normal rats the whole microvasculature was visualized, but in both normal and shocked animals injected at low pressure different patterns could be found, generally showing a highly incomplete visualization of the vascular network. A significant decrease of visualization of both the entire microvasculature and the villous plexus is present in shocked animals when compared to unshocked controls, while no difference in the cryptal plexus visualization was observed. These observations suggest that the cryptal plexus is perfused preferentially during hemorrhagic shock, as a consequence of its peculiar microvascular organization. This may explain the relative resistance of the crypts, compared to villi, to hypoxic injuries in order to sustain endocrine function and the regenerative capability of the mucosa after prolonged hypoperfusion conditions that can lead to villous damage and temporary loss of the intestinal barrier function.

Original languageEnglish (US)
Pages (from-to)710-722
Number of pages13
JournalDigestive Diseases and Sciences
Volume45
Issue number4
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Hemorrhagic Shock
Microvessels
Blood Vessels
Ink
Corrosion
Pressure
Injections
Mucous Membrane
Wounds and Injuries
Intestinal Mucosa
Microspheres
Electron Scanning Microscopy
Shock
Arterial Pressure

Keywords

  • Blood supply
  • Hemorrhagic shock
  • Intestine
  • Microcirculation
  • Vascular corrosion cast

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Intestinal microvascular patterns during hemorrhagic shock. / Morini, Sergio; Yacoub, Wael; Rastellini, Cristiana; Gaudio, Eugenio; Watkins, Simon C.; Cicalese, Luca.

In: Digestive Diseases and Sciences, Vol. 45, No. 4, 2000, p. 710-722.

Research output: Contribution to journalArticle

Morini, S, Yacoub, W, Rastellini, C, Gaudio, E, Watkins, SC & Cicalese, L 2000, 'Intestinal microvascular patterns during hemorrhagic shock', Digestive Diseases and Sciences, vol. 45, no. 4, pp. 710-722. https://doi.org/10.1023/A:1005491509832
Morini, Sergio ; Yacoub, Wael ; Rastellini, Cristiana ; Gaudio, Eugenio ; Watkins, Simon C. ; Cicalese, Luca. / Intestinal microvascular patterns during hemorrhagic shock. In: Digestive Diseases and Sciences. 2000 ; Vol. 45, No. 4. pp. 710-722.
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