Intestinal peptide YY

Ontogeny of gene expression in rat bowel and trophic actions on rat and mouse bowel

Guillermo Gomez, T. Zhang, S. Rajaraman, K. N. Thakore, N. Yanaihara, Courtney Townsend, J. C. Thompson, G. H. Greeley

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was twofold: 1) to characterize the profile of colonic peptide YY (PYY) gene expression in rats and 2) to examine for potential trophic effects of PYY on the intestine in rats and mice. Expression of PYY mRNA (analyzed by Northern blotting and in situ hybridization) and PYY (analyzed by high-performance liquid chromatography and radioimmunoassay) was detected initially at day 17 of gestation in colonic extracts of Sprague-Dawley and Fischer rats. Expression of colonic PYY mRNA increased until 7 days of age and remained at its highest level (approximately twofold greater than the adult level) through the end of the nursing period. After weaning (21 days of age), PYY mRNA levels declined quickly to adult levels. Colonic PYY concentrations followed, in a coordinated manner, with some temporal delay after birth, the increase and decrease of its mRNA. Administration of PYY increased the weight and DNA content of the duodenum significantly in nursing rats and adult mice. In mice, PYY treatment also increased weight and DNA content of the ileum and colon. The trophic effects of PYY were dose related, peptide specific, and independent of species and sex. From these findings, we hypothesize that PYY plays an important role in intestinal development and dietary adaptation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume268
Issue number1 31-1
StatePublished - 1995

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peptide YY
Peptide YY
ontogeny
Gene Expression
gene expression
rats
mice
Messenger RNA
breast feeding
Nursing
Weights and Measures
DNA
Inbred F344 Rats
Weaning
radioimmunoassays
duodenum
Ileum
Duodenum
ileum
Northern blotting

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Agricultural and Biological Sciences(all)

Cite this

Intestinal peptide YY : Ontogeny of gene expression in rat bowel and trophic actions on rat and mouse bowel. / Gomez, Guillermo; Zhang, T.; Rajaraman, S.; Thakore, K. N.; Yanaihara, N.; Townsend, Courtney; Thompson, J. C.; Greeley, G. H.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 268, No. 1 31-1, 1995.

Research output: Contribution to journalArticle

Gomez, Guillermo ; Zhang, T. ; Rajaraman, S. ; Thakore, K. N. ; Yanaihara, N. ; Townsend, Courtney ; Thompson, J. C. ; Greeley, G. H. / Intestinal peptide YY : Ontogeny of gene expression in rat bowel and trophic actions on rat and mouse bowel. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1995 ; Vol. 268, No. 1 31-1.
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