Intestinal phase of pancreatic polypeptide release

The effect of segmental perfusion of the small intestine with various secretagogues

M. Fujimura, T. Khalil, T. Sakamoto, G. H. Greeley, Courtney Townsend, J. C. Thompson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The purpose of this study was to examine the effect of various nutrients perfused selectively into isolated sections of the small intestine on the release of pancreatic polypeptide (PP). Six dogs were prepared with chronic gastric, duodenal, and three permanent intestinal fistulas (one distal to the ligament of Treitz, one in the terminal ileum, and one halfway between). After a 24-h fast, the duodenum was perfused for 45 min (200 ml/h) via the duodenal limb with HCl (30 mM), an amino acid solution (50 mM), or sodium oleate (NaO, 40 mM). In separate studies, the jejunal and ileal segments were isolated by inflating two balloon catheters via the intestinal fistula, and these segments were perfused as described earlier. Plasma PP, cholecystokinin (CCK)-33, and secretin levels were measured. Results of this study showed that the perfusion of all segments of the small intestine with amino acid caused a significant elevation of plasma PP levels. Perfusion of the duodenum and jejunum but not the ileum with a fatty acid resulted in a significant elevation of plasma levels of PP. Perfusion of HCl into any segment did not affect plasma levels of PP. This study shows that PP is released by the presence of nutrients in all segments of the small intestine, and that a PP elevation was always accompanied with a rise in plasma levels of CCK-33.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalPancreas
Volume5
Issue number2
StatePublished - 1990

Fingerprint

Pancreatic Polypeptide
Small Intestine
Perfusion
Intestinal Fistula
Cholecystokinin
Ileum
Duodenum
Amino Acids
Food
Secretin
Jejunum
Ligaments
Stomach
Fatty Acids
Catheters
Extremities
Dogs

Keywords

  • Amino acids
  • Dogs
  • Hydrochloride
  • Intestinal perfusion
  • Pancreatic polypeptide
  • Sodium oleate

ASJC Scopus subject areas

  • Endocrinology
  • Gastroenterology

Cite this

Intestinal phase of pancreatic polypeptide release : The effect of segmental perfusion of the small intestine with various secretagogues. / Fujimura, M.; Khalil, T.; Sakamoto, T.; Greeley, G. H.; Townsend, Courtney; Thompson, J. C.

In: Pancreas, Vol. 5, No. 2, 1990, p. 183-187.

Research output: Contribution to journalArticle

Fujimura, M. ; Khalil, T. ; Sakamoto, T. ; Greeley, G. H. ; Townsend, Courtney ; Thompson, J. C. / Intestinal phase of pancreatic polypeptide release : The effect of segmental perfusion of the small intestine with various secretagogues. In: Pancreas. 1990 ; Vol. 5, No. 2. pp. 183-187.
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