Intragraft Gene Expression Profile During Acute Cellular Rejection in Clinical Small Bowel Transplantation

A Case Report

S. P. Bradley, M. Pahari, G. Elias, M. E. Uknis, M. V. Misra, Cristiana Rastellini, Luca Cicalese

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Acute cellular rejection (ACR) is a common complication of small bowel transplantation (SBTx) and the major cause of graft loss. However, little is known regarding the genetic graft response to ACR in clinical transplants. In this study, we have determined a genetic expression profile of intestinal graft response to ACR after living related (LR) SBTx. Results: By identifying the expression profiles of reported markers of rejection we were able to identify 57 genes that had significantly increased (more than twofold) expression in response to ACR. Known markers of rejection identified: MMP-9, MMP-2, VIP, IFNγ, IL-2R, MADCAM-1, HSP-60, and HSP-70 all had greater than twofold increased expression after ACR diagnosed (week 3 to week 6). The newly identified genes were: IFI27, EPST11, APAF1, LAP3, STK6, and MDK. Conclusion: Newly identified up-regulated genes in response to ACR in small bowel graft are involved in the immune response, cell adhesion, neurogenesis, cell division and proliferation, DNA replication/repair, protein ubiqutin/proteolysis, and apoptosis. TNFα up-regulated early at week 2 biopsy may be an early genetic marker of ACR in SBTx.

Original languageEnglish (US)
Pages (from-to)1740-1741
Number of pages2
JournalTransplantation Proceedings
Volume38
Issue number6
DOIs
StatePublished - Jul 2006
Externally publishedYes

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Transcriptome
Transplantation
Transplants
Matrix Metalloproteinases
Genes
Neurogenesis
DNA Replication
Genetic Markers
Cell Adhesion
DNA Repair
Cell Division
Proteolysis
Cell Proliferation
Apoptosis
Biopsy
Proteins

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Intragraft Gene Expression Profile During Acute Cellular Rejection in Clinical Small Bowel Transplantation : A Case Report. / Bradley, S. P.; Pahari, M.; Elias, G.; Uknis, M. E.; Misra, M. V.; Rastellini, Cristiana; Cicalese, Luca.

In: Transplantation Proceedings, Vol. 38, No. 6, 07.2006, p. 1740-1741.

Research output: Contribution to journalArticle

Bradley, S. P. ; Pahari, M. ; Elias, G. ; Uknis, M. E. ; Misra, M. V. ; Rastellini, Cristiana ; Cicalese, Luca. / Intragraft Gene Expression Profile During Acute Cellular Rejection in Clinical Small Bowel Transplantation : A Case Report. In: Transplantation Proceedings. 2006 ; Vol. 38, No. 6. pp. 1740-1741.
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