Intraoperative touch preparation for sentinel lymph node biopsy

A 4-year experience

Ronda S. Henry-Tillman, Soheila Korourian, Isabel T. Rubio, Anita T. Johnson, Anne T. Mancino, Nicole Massol, Lanette F. Smith, Kent C. Westbrook, Vicki Klimberg

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Background: The optimal technique for intraoperative pathologic examination of sentinel lymph nodes (SLNs) is still controversial. Recent small series report sensitivity between 60% and 100% for various techniques. The aim of this study was to evaluate our long-term experience with touch preparation cytology (TPC) and frozen section (FS) in the intraoperative examination of SLNs for breast cancer. Methods: A total of 247 patients with operable breast cancer underwent an SLN biopsy for staging of the axilla. The SLN was identified by 99mTc-labeled sulfur colloid unfiltered dye, blue dye, or both and dissected, and then intraoperative TPC or FS and permanent section, or both, were performed. Results: A total of 479 SLNs were submitted for TPC and permanent hematoxylin and eosin. A total of 68 SLNs were positive by hematoxylin and eosin; 65 SLNs were positive by TPC, with a false-negative rate of 5.8%. The sensitivity for TPC was 94.2%, with a false-positive rate of 0.2%. A total of 165 SLNs were submitted for FS, with a sensitivity of 85.7% and a specificity of 98.6%. The false-positive rate was 1.4%, with a false-negative rate of 15.8%. Conclusions: In a large series, TPC is as accurate as FS but is simpler and faster in the detection of intraoperative metastasis in SLNs for breast cancer.

Original languageEnglish (US)
Pages (from-to)333-339
Number of pages7
JournalAnnals of Surgical Oncology
Volume9
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

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Sentinel Lymph Node Biopsy
Touch
Cell Biology
Frozen Sections
Hematoxylin
Eosine Yellowish-(YS)
Breast Neoplasms
Coloring Agents
Axilla
Sentinel Lymph Node
Colloids
Sulfur
Neoplasm Metastasis

Keywords

  • Accuracy
  • Breast cancer
  • Frozen section
  • Sentinel lymph node
  • Touch preparation cytology

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Henry-Tillman, R. S., Korourian, S., Rubio, I. T., Johnson, A. T., Mancino, A. T., Massol, N., ... Klimberg, V. (2002). Intraoperative touch preparation for sentinel lymph node biopsy: A 4-year experience. Annals of Surgical Oncology, 9(4), 333-339. https://doi.org/10.1245/aso.2002.9.4.333

Intraoperative touch preparation for sentinel lymph node biopsy : A 4-year experience. / Henry-Tillman, Ronda S.; Korourian, Soheila; Rubio, Isabel T.; Johnson, Anita T.; Mancino, Anne T.; Massol, Nicole; Smith, Lanette F.; Westbrook, Kent C.; Klimberg, Vicki.

In: Annals of Surgical Oncology, Vol. 9, No. 4, 2002, p. 333-339.

Research output: Contribution to journalArticle

Henry-Tillman, RS, Korourian, S, Rubio, IT, Johnson, AT, Mancino, AT, Massol, N, Smith, LF, Westbrook, KC & Klimberg, V 2002, 'Intraoperative touch preparation for sentinel lymph node biopsy: A 4-year experience', Annals of Surgical Oncology, vol. 9, no. 4, pp. 333-339. https://doi.org/10.1245/aso.2002.9.4.333
Henry-Tillman RS, Korourian S, Rubio IT, Johnson AT, Mancino AT, Massol N et al. Intraoperative touch preparation for sentinel lymph node biopsy: A 4-year experience. Annals of Surgical Oncology. 2002;9(4):333-339. https://doi.org/10.1245/aso.2002.9.4.333
Henry-Tillman, Ronda S. ; Korourian, Soheila ; Rubio, Isabel T. ; Johnson, Anita T. ; Mancino, Anne T. ; Massol, Nicole ; Smith, Lanette F. ; Westbrook, Kent C. ; Klimberg, Vicki. / Intraoperative touch preparation for sentinel lymph node biopsy : A 4-year experience. In: Annals of Surgical Oncology. 2002 ; Vol. 9, No. 4. pp. 333-339.
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abstract = "Background: The optimal technique for intraoperative pathologic examination of sentinel lymph nodes (SLNs) is still controversial. Recent small series report sensitivity between 60{\%} and 100{\%} for various techniques. The aim of this study was to evaluate our long-term experience with touch preparation cytology (TPC) and frozen section (FS) in the intraoperative examination of SLNs for breast cancer. Methods: A total of 247 patients with operable breast cancer underwent an SLN biopsy for staging of the axilla. The SLN was identified by 99mTc-labeled sulfur colloid unfiltered dye, blue dye, or both and dissected, and then intraoperative TPC or FS and permanent section, or both, were performed. Results: A total of 479 SLNs were submitted for TPC and permanent hematoxylin and eosin. A total of 68 SLNs were positive by hematoxylin and eosin; 65 SLNs were positive by TPC, with a false-negative rate of 5.8{\%}. The sensitivity for TPC was 94.2{\%}, with a false-positive rate of 0.2{\%}. A total of 165 SLNs were submitted for FS, with a sensitivity of 85.7{\%} and a specificity of 98.6{\%}. The false-positive rate was 1.4{\%}, with a false-negative rate of 15.8{\%}. Conclusions: In a large series, TPC is as accurate as FS but is simpler and faster in the detection of intraoperative metastasis in SLNs for breast cancer.",
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