Intrapleural low-molecular-weight urokinase or tissue plasminogen activator versus single-chain urokinase in tetracycline-induced pleural loculation in rabbits

Steven Idell, Ali Azghani, Shande Chen, Kathy Koenig, Andrew Mazar, Lalitha Kodandapani, Khalil Bdeir, Douglas Cines, Irina Kulikovskaya, Timothy Allen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The authors compared the ability of a single dose of the proenzyme single-chain urokinase (scuPA), low-molecular-weight urokinase, tissue plasminogen activator (tPA), or a mutant site-inactive scuPA to resolve intrapleural loculations at 72 to 96 hours after tetracycline-induced pleural injury in rabbits. Both scuPA and tPA reversed loculations at 96 hours after injury P ≤ .001, whereas low-molecular-weight urokinase and the scuPA mutant were ineffective. scuPA and tPA generated inhibitor complexes, induced fibrinolytic activity, and quenched plasminogen activator-1 activity in pleural fluids. The authors conclude that scuPA reverses loculations as effectively as tPA at clinically applied intrapleural doses, whereas low-molecular-weight urokinase was ineffective.

Original languageEnglish (US)
Pages (from-to)419-440
Number of pages22
JournalExperimental Lung Research
Volume33
Issue number8-9
DOIs
StatePublished - Oct 2007
Externally publishedYes

Fingerprint

Urokinase-Type Plasminogen Activator
Tissue Plasminogen Activator
Tetracycline
Molecular Weight
Molecular weight
Rabbits
Plasminogen Inactivators
Enzyme Precursors
Plasminogen Activators
Wounds and Injuries
Fluids

Keywords

  • Fibrinolysis
  • Loculation
  • Pleurodesis
  • Tissue plasminogen activator
  • Urokinase

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Intrapleural low-molecular-weight urokinase or tissue plasminogen activator versus single-chain urokinase in tetracycline-induced pleural loculation in rabbits. / Idell, Steven; Azghani, Ali; Chen, Shande; Koenig, Kathy; Mazar, Andrew; Kodandapani, Lalitha; Bdeir, Khalil; Cines, Douglas; Kulikovskaya, Irina; Allen, Timothy.

In: Experimental Lung Research, Vol. 33, No. 8-9, 10.2007, p. 419-440.

Research output: Contribution to journalArticle

Idell, S, Azghani, A, Chen, S, Koenig, K, Mazar, A, Kodandapani, L, Bdeir, K, Cines, D, Kulikovskaya, I & Allen, T 2007, 'Intrapleural low-molecular-weight urokinase or tissue plasminogen activator versus single-chain urokinase in tetracycline-induced pleural loculation in rabbits', Experimental Lung Research, vol. 33, no. 8-9, pp. 419-440. https://doi.org/10.1080/01902140701703333
Idell, Steven ; Azghani, Ali ; Chen, Shande ; Koenig, Kathy ; Mazar, Andrew ; Kodandapani, Lalitha ; Bdeir, Khalil ; Cines, Douglas ; Kulikovskaya, Irina ; Allen, Timothy. / Intrapleural low-molecular-weight urokinase or tissue plasminogen activator versus single-chain urokinase in tetracycline-induced pleural loculation in rabbits. In: Experimental Lung Research. 2007 ; Vol. 33, No. 8-9. pp. 419-440.
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