Intraspinal sprouting of calcitonin gene-related peptide containing primary afferents after deafferentation in the rat

Daniel L. McNeill, Susan M. Carlton, Claire E. Hulsebosch

    Research output: Contribution to journalArticle

    71 Citations (Scopus)

    Abstract

    The occurrence of sprouting in the spinal cord in response to denervation has been a subject of debate. To test for sprouting of primary afferent fibers after denervation, rats were unilaterally deafferented for 35 days (chronic side) by dorsal rhizotomies performed from T2 to T8 and T10 to L5, thus isolating or sparing the T9 root. The contralateral T9 root was spared by a similar surgery 5 days (acute side) prior to sacrifice. The survival time on the chronic side presumably allows intraspinal sprouting of T9 primary afferents to occur whereas the time on the acute side does not. To test for sprouting of primary afferents, it is necessary to identify these nerve processes. Calcitonin gene-related peptide (CGRP) immunoreactivity has been localized to a subpopulation of primary afferent nerve processes and their terminals within the dorsal horn. Therefore, immunohistochemical methods were used to determine the distribution of CGRP immunoreactivity in laminae I and II on both sides of the spinal cord. Using image analysis, there was an increase of 153 to 704% in the density of CGRP immunoreaction product on the chronic side compared to the acute side in the spared segment. This difference is statistically significant. Furthermore, the increased density on the chronic side extended two segments cranial and two segments caudal to the spared root segment. No difference was found in the laminar distribution between sides. These data support the hypothesis of primary afferent sprouting following spinal cord denervation.

    Original languageEnglish (US)
    Pages (from-to)321-329
    Number of pages9
    JournalExperimental Neurology
    Volume114
    Issue number3
    DOIs
    StatePublished - 1991

    Fingerprint

    Calcitonin Gene-Related Peptide
    Denervation
    Spinal Cord
    Substantia Gelatinosa
    Rhizotomy
    Ambulatory Surgical Procedures
    Spinal Cord Dorsal Horn

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Neurology

    Cite this

    Intraspinal sprouting of calcitonin gene-related peptide containing primary afferents after deafferentation in the rat. / McNeill, Daniel L.; Carlton, Susan M.; Hulsebosch, Claire E.

    In: Experimental Neurology, Vol. 114, No. 3, 1991, p. 321-329.

    Research output: Contribution to journalArticle

    McNeill, Daniel L. ; Carlton, Susan M. ; Hulsebosch, Claire E. / Intraspinal sprouting of calcitonin gene-related peptide containing primary afferents after deafferentation in the rat. In: Experimental Neurology. 1991 ; Vol. 114, No. 3. pp. 321-329.
    @article{5486a997b70f42758cc7bde22f8fdb06,
    title = "Intraspinal sprouting of calcitonin gene-related peptide containing primary afferents after deafferentation in the rat",
    abstract = "The occurrence of sprouting in the spinal cord in response to denervation has been a subject of debate. To test for sprouting of primary afferent fibers after denervation, rats were unilaterally deafferented for 35 days (chronic side) by dorsal rhizotomies performed from T2 to T8 and T10 to L5, thus isolating or sparing the T9 root. The contralateral T9 root was spared by a similar surgery 5 days (acute side) prior to sacrifice. The survival time on the chronic side presumably allows intraspinal sprouting of T9 primary afferents to occur whereas the time on the acute side does not. To test for sprouting of primary afferents, it is necessary to identify these nerve processes. Calcitonin gene-related peptide (CGRP) immunoreactivity has been localized to a subpopulation of primary afferent nerve processes and their terminals within the dorsal horn. Therefore, immunohistochemical methods were used to determine the distribution of CGRP immunoreactivity in laminae I and II on both sides of the spinal cord. Using image analysis, there was an increase of 153 to 704{\%} in the density of CGRP immunoreaction product on the chronic side compared to the acute side in the spared segment. This difference is statistically significant. Furthermore, the increased density on the chronic side extended two segments cranial and two segments caudal to the spared root segment. No difference was found in the laminar distribution between sides. These data support the hypothesis of primary afferent sprouting following spinal cord denervation.",
    author = "McNeill, {Daniel L.} and Carlton, {Susan M.} and Hulsebosch, {Claire E.}",
    year = "1991",
    doi = "10.1016/0014-4886(91)90158-9",
    language = "English (US)",
    volume = "114",
    pages = "321--329",
    journal = "Experimental Neurology",
    issn = "0014-4886",
    publisher = "Academic Press Inc.",
    number = "3",

    }

    TY - JOUR

    T1 - Intraspinal sprouting of calcitonin gene-related peptide containing primary afferents after deafferentation in the rat

    AU - McNeill, Daniel L.

    AU - Carlton, Susan M.

    AU - Hulsebosch, Claire E.

    PY - 1991

    Y1 - 1991

    N2 - The occurrence of sprouting in the spinal cord in response to denervation has been a subject of debate. To test for sprouting of primary afferent fibers after denervation, rats were unilaterally deafferented for 35 days (chronic side) by dorsal rhizotomies performed from T2 to T8 and T10 to L5, thus isolating or sparing the T9 root. The contralateral T9 root was spared by a similar surgery 5 days (acute side) prior to sacrifice. The survival time on the chronic side presumably allows intraspinal sprouting of T9 primary afferents to occur whereas the time on the acute side does not. To test for sprouting of primary afferents, it is necessary to identify these nerve processes. Calcitonin gene-related peptide (CGRP) immunoreactivity has been localized to a subpopulation of primary afferent nerve processes and their terminals within the dorsal horn. Therefore, immunohistochemical methods were used to determine the distribution of CGRP immunoreactivity in laminae I and II on both sides of the spinal cord. Using image analysis, there was an increase of 153 to 704% in the density of CGRP immunoreaction product on the chronic side compared to the acute side in the spared segment. This difference is statistically significant. Furthermore, the increased density on the chronic side extended two segments cranial and two segments caudal to the spared root segment. No difference was found in the laminar distribution between sides. These data support the hypothesis of primary afferent sprouting following spinal cord denervation.

    AB - The occurrence of sprouting in the spinal cord in response to denervation has been a subject of debate. To test for sprouting of primary afferent fibers after denervation, rats were unilaterally deafferented for 35 days (chronic side) by dorsal rhizotomies performed from T2 to T8 and T10 to L5, thus isolating or sparing the T9 root. The contralateral T9 root was spared by a similar surgery 5 days (acute side) prior to sacrifice. The survival time on the chronic side presumably allows intraspinal sprouting of T9 primary afferents to occur whereas the time on the acute side does not. To test for sprouting of primary afferents, it is necessary to identify these nerve processes. Calcitonin gene-related peptide (CGRP) immunoreactivity has been localized to a subpopulation of primary afferent nerve processes and their terminals within the dorsal horn. Therefore, immunohistochemical methods were used to determine the distribution of CGRP immunoreactivity in laminae I and II on both sides of the spinal cord. Using image analysis, there was an increase of 153 to 704% in the density of CGRP immunoreaction product on the chronic side compared to the acute side in the spared segment. This difference is statistically significant. Furthermore, the increased density on the chronic side extended two segments cranial and two segments caudal to the spared root segment. No difference was found in the laminar distribution between sides. These data support the hypothesis of primary afferent sprouting following spinal cord denervation.

    UR - http://www.scopus.com/inward/record.url?scp=0026352313&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0026352313&partnerID=8YFLogxK

    U2 - 10.1016/0014-4886(91)90158-9

    DO - 10.1016/0014-4886(91)90158-9

    M3 - Article

    C2 - 1748206

    AN - SCOPUS:0026352313

    VL - 114

    SP - 321

    EP - 329

    JO - Experimental Neurology

    JF - Experimental Neurology

    SN - 0014-4886

    IS - 3

    ER -