Intravascular persistence of Anaplasma platys, Ehrlichia chaffeensis, and Ehrlichia ewingii DNA in the blood of a dog and two family members

Edward B. Breitschwerdt, Barbara C. Hegarty, Barbara A. Qurollo, Tais Saito, Ricardo G. Maggi, Lucas Blanton, Donald Bouyer

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: Anaplasmosis, caused by Anaplasma phagocytophilum and Anaplasma platys, and ehrlichiosis, caused by Ehrlichia chaffeensis, Ehrlichia ewingii, the Panola Mountain Ehrlichia and Ehrlichia muris-like pathogens have been identified as emerging tick borne infectious diseases in dogs and human patients. Persistent intravascular infection with these bacteria is well documented in dogs, but is less well documented in human beings. Methods. Serology and PCR targeting multiple microbial genes, followed by DNA sequencing, was used to test sequential blood samples. Tissue culture isolation was attempted in two laboratories. Results: A. platys, E. chaffeensis, and E. ewingii DNA was amplified from two Anaplasma and Ehrlichia seronegative family members and their dog, all lacking typical symptoms of anaplasmosis or ehrlichiosis. Following treatment with doxycycline, the dog and mother were Anaplasma and Ehrlichia spp. PCR negative. Conclusions: Sequential PCR testing provided molecular evidence supporting intravascular persistence of A. platys and Ehrlichia spp. in two humans and their dog. Diagnosticians and clinicians should consider the potential for co-infections due to these tick borne organisms.

Original languageEnglish (US)
Article number298
JournalParasites and Vectors
Volume7
Issue number1
DOIs
StatePublished - Jul 1 2014

Fingerprint

Ehrlichia chaffeensis
Anaplasma
Ehrlichia
Dogs
DNA
Anaplasmosis
Ehrlichiosis
Polymerase Chain Reaction
Microbial Genes
Anaplasma phagocytophilum
Tick-Borne Diseases
Doxycycline
Hematologic Tests
Ticks
Serology
Coinfection
DNA Sequence Analysis
Communicable Diseases
Mothers
Bacteria

Keywords

  • Anaplasma
  • DNA sequencing
  • Ehrlichia
  • PCR
  • Rickettsemia

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Medicine(all)

Cite this

Intravascular persistence of Anaplasma platys, Ehrlichia chaffeensis, and Ehrlichia ewingii DNA in the blood of a dog and two family members. / Breitschwerdt, Edward B.; Hegarty, Barbara C.; Qurollo, Barbara A.; Saito, Tais; Maggi, Ricardo G.; Blanton, Lucas; Bouyer, Donald.

In: Parasites and Vectors, Vol. 7, No. 1, 298, 01.07.2014.

Research output: Contribution to journalArticle

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