Introduction of liposome sequestered human hexosaminidase A and ferritin into lysosomes of mouse macrophages

J. E. Wiktorowicz, P. S. Baur, Satish Srivastava

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Homogeneous hexosaminidase A and ferritin were sequestered in liposomes consisting of lecithin (phosphatidyl choline), dicetyl phosphate and cholesterol in the molar ratio of 7 : 2 : 1. These liposomes were coated with heat aggregated IgG and presented to mouse macrophages harvested from the peritoneal cavity. The infiltration of macrophages in the peritoneal cavity was accomplished by injection of 5% oyster glycogen. The cells were pelleted, fixed, embedded, and sections were examined under the electron microscope. Evidence is presented ted that the liposomes coated with heat aggregated IgG, are phagocytised by the macrophages and enter into the lysosomes. The ferritin is deposited exclusively in the lysosomes, a result that enhances the efficacy of liposomes as carriers of enzymes in enzyme replacement therapy in lysosomal storage disorders.

Original languageEnglish (US)
Title of host publicationCytobiologie
Pages401-411
Number of pages11
Volume14
Edition3
StatePublished - 1977

Fingerprint

Hexosaminidase A
Ferritins
Lysosomes
Liposomes
Macrophages
Peritoneal Cavity
Immunoglobulin G
Hot Temperature
Enzyme Replacement Therapy
Ostreidae
Phosphorylcholine
Lecithins
Phosphatidylcholines
Glycogen
Cholesterol
Electrons
Injections
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Wiktorowicz, J. E., Baur, P. S., & Srivastava, S. (1977). Introduction of liposome sequestered human hexosaminidase A and ferritin into lysosomes of mouse macrophages. In Cytobiologie (3 ed., Vol. 14, pp. 401-411)

Introduction of liposome sequestered human hexosaminidase A and ferritin into lysosomes of mouse macrophages. / Wiktorowicz, J. E.; Baur, P. S.; Srivastava, Satish.

Cytobiologie. Vol. 14 3. ed. 1977. p. 401-411.

Research output: Chapter in Book/Report/Conference proceedingChapter

Wiktorowicz, JE, Baur, PS & Srivastava, S 1977, Introduction of liposome sequestered human hexosaminidase A and ferritin into lysosomes of mouse macrophages. in Cytobiologie. 3 edn, vol. 14, pp. 401-411.
Wiktorowicz JE, Baur PS, Srivastava S. Introduction of liposome sequestered human hexosaminidase A and ferritin into lysosomes of mouse macrophages. In Cytobiologie. 3 ed. Vol. 14. 1977. p. 401-411
Wiktorowicz, J. E. ; Baur, P. S. ; Srivastava, Satish. / Introduction of liposome sequestered human hexosaminidase A and ferritin into lysosomes of mouse macrophages. Cytobiologie. Vol. 14 3. ed. 1977. pp. 401-411
@inbook{017f8c61198c4927a82641625c7a34c0,
title = "Introduction of liposome sequestered human hexosaminidase A and ferritin into lysosomes of mouse macrophages",
abstract = "Homogeneous hexosaminidase A and ferritin were sequestered in liposomes consisting of lecithin (phosphatidyl choline), dicetyl phosphate and cholesterol in the molar ratio of 7 : 2 : 1. These liposomes were coated with heat aggregated IgG and presented to mouse macrophages harvested from the peritoneal cavity. The infiltration of macrophages in the peritoneal cavity was accomplished by injection of 5{\%} oyster glycogen. The cells were pelleted, fixed, embedded, and sections were examined under the electron microscope. Evidence is presented ted that the liposomes coated with heat aggregated IgG, are phagocytised by the macrophages and enter into the lysosomes. The ferritin is deposited exclusively in the lysosomes, a result that enhances the efficacy of liposomes as carriers of enzymes in enzyme replacement therapy in lysosomal storage disorders.",
author = "Wiktorowicz, {J. E.} and Baur, {P. S.} and Satish Srivastava",
year = "1977",
language = "English (US)",
volume = "14",
pages = "401--411",
booktitle = "Cytobiologie",
edition = "3",

}

TY - CHAP

T1 - Introduction of liposome sequestered human hexosaminidase A and ferritin into lysosomes of mouse macrophages

AU - Wiktorowicz, J. E.

AU - Baur, P. S.

AU - Srivastava, Satish

PY - 1977

Y1 - 1977

N2 - Homogeneous hexosaminidase A and ferritin were sequestered in liposomes consisting of lecithin (phosphatidyl choline), dicetyl phosphate and cholesterol in the molar ratio of 7 : 2 : 1. These liposomes were coated with heat aggregated IgG and presented to mouse macrophages harvested from the peritoneal cavity. The infiltration of macrophages in the peritoneal cavity was accomplished by injection of 5% oyster glycogen. The cells were pelleted, fixed, embedded, and sections were examined under the electron microscope. Evidence is presented ted that the liposomes coated with heat aggregated IgG, are phagocytised by the macrophages and enter into the lysosomes. The ferritin is deposited exclusively in the lysosomes, a result that enhances the efficacy of liposomes as carriers of enzymes in enzyme replacement therapy in lysosomal storage disorders.

AB - Homogeneous hexosaminidase A and ferritin were sequestered in liposomes consisting of lecithin (phosphatidyl choline), dicetyl phosphate and cholesterol in the molar ratio of 7 : 2 : 1. These liposomes were coated with heat aggregated IgG and presented to mouse macrophages harvested from the peritoneal cavity. The infiltration of macrophages in the peritoneal cavity was accomplished by injection of 5% oyster glycogen. The cells were pelleted, fixed, embedded, and sections were examined under the electron microscope. Evidence is presented ted that the liposomes coated with heat aggregated IgG, are phagocytised by the macrophages and enter into the lysosomes. The ferritin is deposited exclusively in the lysosomes, a result that enhances the efficacy of liposomes as carriers of enzymes in enzyme replacement therapy in lysosomal storage disorders.

UR - http://www.scopus.com/inward/record.url?scp=0017347895&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017347895&partnerID=8YFLogxK

M3 - Chapter

AN - SCOPUS:0017347895

VL - 14

SP - 401

EP - 411

BT - Cytobiologie

ER -