Inverted-colloidal-crystal hydrogel matrices as three-dimensional cell scaffolds

Yongjun Zhang, Shaopeng Wang, Mohammad Eghtedari, Massoud Motamedi, Nicholas A. Kotov

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Successful engineering of functional tissues requires the development of three-dimensional (3D) scaffolds that can provide an optimum microenviromnent for tissue growth and regeneration. A new class of 3D scaffolds with a high degree of organization and unique topography is fabricated from polyacrylamide hydrogel. The hydrogel matrix is molded by inverted colloidal crystals made from 104 μm poly(methyl methacrylate) spheres. The topography of the scaffold can be described as hexagonally packed 97 μm spherical cavities interconnected by a network of channels. The scale of the long-range ordering of the cavities exceeds several millimeters. In contrast to analogous material in the bulk, hydrogel shaped as an inverted opal exhibits much higher swelling ratios; its swelling kinetics is an order of magnitude faster as well. The engineered scaffold possesses desirable mechanical and optical properties that can facilitate tissue regeneration while allowing for continuous high-resolution optical monitoring of cell proliferation and cell-cell interaction within the scaffold. The scaffold biocompatibility as well as cellular growth and infiltration within the scaffold were observed for two distinct human cell lines which were seeded on the scaffold and were tracked microscopically up to a depth of 250 μm within the scaffold for a duration of up to five weeks. Ease of production, a unique 3D structure, biocompatibility, and optical transparency make this new type of hydrogel scaffold suitable for most challenging tasks in tissue engineering.

Original languageEnglish (US)
Pages (from-to)725-731
Number of pages7
JournalAdvanced Functional Materials
Volume15
Issue number5
DOIs
StatePublished - May 2005

Fingerprint

Hydrogel
Hydrogels
Scaffolds
biocompatibility
regeneration
swelling
Crystals
topography
matrices
cells
crystals
Scaffolds (biology)
cavities
tissue engineering
infiltration
cultured cells
polymethyl methacrylate
Biocompatibility
Topography
Swelling

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Materials Science(all)
  • Condensed Matter Physics
  • Physics and Astronomy (miscellaneous)

Cite this

Inverted-colloidal-crystal hydrogel matrices as three-dimensional cell scaffolds. / Zhang, Yongjun; Wang, Shaopeng; Eghtedari, Mohammad; Motamedi, Massoud; Kotov, Nicholas A.

In: Advanced Functional Materials, Vol. 15, No. 5, 05.2005, p. 725-731.

Research output: Contribution to journalArticle

Zhang, Yongjun ; Wang, Shaopeng ; Eghtedari, Mohammad ; Motamedi, Massoud ; Kotov, Nicholas A. / Inverted-colloidal-crystal hydrogel matrices as three-dimensional cell scaffolds. In: Advanced Functional Materials. 2005 ; Vol. 15, No. 5. pp. 725-731.
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