Inverted colloidal crystals as three-dimensional cell scaffolds

Nicholas A. Kotov, Yuanfang Liu, Shaopeng Wang, Colin Cumming, Mohammad Eghtedari, Gracie Vargas, Massoud Motamedi, Joan Nichols, Joaquin Cortiella

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

A new type of three-dimensional scaffold with inverted colloidal crystal geometry for the investigation of topological effects in cell cultures is introduced in this publication. The scaffolds are made by infiltration of the hexagonal crystal lattice of polystyrene spheres with sol-gel formulation and subsequent annealing. It possesses a relatively high degree of order among existing cell scaffolds and affords tight control over the scaffold porosity and tissue organization. The prepared scaffolds can be a convenient system for the investigation of cell-cell and cell-matrix interactions. Their biocompatibility is demonstrated for human hepatocellular carcinoma HEP G2 and human bone marrow HS-5 cell cultures. A preliminary effect of the scaffold topology on cell proliferation is observed. HEP G2 hepatocytes form a large number of 10-15 cell colonies on scaffolds made from 75-μm spheres, while their number diminishes for scaffolds from 10- and 160-μm spheres. Under similar conditions, HS-5 forms smaller colonies consisting of three to four cells in 90-μm cavities.

Original languageEnglish (US)
Pages (from-to)7887-7892
Number of pages6
JournalLangmuir
Volume20
Issue number19
DOIs
StatePublished - Sep 14 2004

Fingerprint

Scaffolds
Crystals
cells
crystals
Cell culture
Tissue Scaffolds
bone marrow
Polystyrenes
Cell proliferation
biocompatibility
infiltration
Scaffolds (biology)
Biocompatibility
Infiltration
crystal lattices
Crystal lattices
Sol-gels
polystyrene
Bone
topology

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Kotov, N. A., Liu, Y., Wang, S., Cumming, C., Eghtedari, M., Vargas, G., ... Cortiella, J. (2004). Inverted colloidal crystals as three-dimensional cell scaffolds. Langmuir, 20(19), 7887-7892. https://doi.org/10.1021/la049958o

Inverted colloidal crystals as three-dimensional cell scaffolds. / Kotov, Nicholas A.; Liu, Yuanfang; Wang, Shaopeng; Cumming, Colin; Eghtedari, Mohammad; Vargas, Gracie; Motamedi, Massoud; Nichols, Joan; Cortiella, Joaquin.

In: Langmuir, Vol. 20, No. 19, 14.09.2004, p. 7887-7892.

Research output: Contribution to journalArticle

Kotov NA, Liu Y, Wang S, Cumming C, Eghtedari M, Vargas G et al. Inverted colloidal crystals as three-dimensional cell scaffolds. Langmuir. 2004 Sep 14;20(19):7887-7892. https://doi.org/10.1021/la049958o
Kotov, Nicholas A. ; Liu, Yuanfang ; Wang, Shaopeng ; Cumming, Colin ; Eghtedari, Mohammad ; Vargas, Gracie ; Motamedi, Massoud ; Nichols, Joan ; Cortiella, Joaquin. / Inverted colloidal crystals as three-dimensional cell scaffolds. In: Langmuir. 2004 ; Vol. 20, No. 19. pp. 7887-7892.
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