Abstract
A new type of three-dimensional scaffold with inverted colloidal crystal geometry for the investigation of topological effects in cell cultures is introduced in this publication. The scaffolds are made by infiltration of the hexagonal crystal lattice of polystyrene spheres with sol-gel formulation and subsequent annealing. It possesses a relatively high degree of order among existing cell scaffolds and affords tight control over the scaffold porosity and tissue organization. The prepared scaffolds can be a convenient system for the investigation of cell-cell and cell-matrix interactions. Their biocompatibility is demonstrated for human hepatocellular carcinoma HEP G2 and human bone marrow HS-5 cell cultures. A preliminary effect of the scaffold topology on cell proliferation is observed. HEP G2 hepatocytes form a large number of 10-15 cell colonies on scaffolds made from 75-μm spheres, while their number diminishes for scaffolds from 10- and 160-μm spheres. Under similar conditions, HS-5 forms smaller colonies consisting of three to four cells in 90-μm cavities.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7887-7892 |
| Number of pages | 6 |
| Journal | Langmuir |
| Volume | 20 |
| Issue number | 19 |
| DOIs | |
| State | Published - Sep 14 2004 |
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ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Cite this
Inverted colloidal crystals as three-dimensional cell scaffolds. / Kotov, Nicholas A.; Liu, Yuanfang; Wang, Shaopeng; Cumming, Colin; Eghtedari, Mohammad; Vargas, Gracie; Motamedi, Massoud; Nichols, Joan; Cortiella, Joaquin.
In: Langmuir, Vol. 20, No. 19, 14.09.2004, p. 7887-7892.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inverted colloidal crystals as three-dimensional cell scaffolds
AU - Kotov, Nicholas A.
AU - Liu, Yuanfang
AU - Wang, Shaopeng
AU - Cumming, Colin
AU - Eghtedari, Mohammad
AU - Vargas, Gracie
AU - Motamedi, Massoud
AU - Nichols, Joan
AU - Cortiella, Joaquin
PY - 2004/9/14
Y1 - 2004/9/14
N2 - A new type of three-dimensional scaffold with inverted colloidal crystal geometry for the investigation of topological effects in cell cultures is introduced in this publication. The scaffolds are made by infiltration of the hexagonal crystal lattice of polystyrene spheres with sol-gel formulation and subsequent annealing. It possesses a relatively high degree of order among existing cell scaffolds and affords tight control over the scaffold porosity and tissue organization. The prepared scaffolds can be a convenient system for the investigation of cell-cell and cell-matrix interactions. Their biocompatibility is demonstrated for human hepatocellular carcinoma HEP G2 and human bone marrow HS-5 cell cultures. A preliminary effect of the scaffold topology on cell proliferation is observed. HEP G2 hepatocytes form a large number of 10-15 cell colonies on scaffolds made from 75-μm spheres, while their number diminishes for scaffolds from 10- and 160-μm spheres. Under similar conditions, HS-5 forms smaller colonies consisting of three to four cells in 90-μm cavities.
AB - A new type of three-dimensional scaffold with inverted colloidal crystal geometry for the investigation of topological effects in cell cultures is introduced in this publication. The scaffolds are made by infiltration of the hexagonal crystal lattice of polystyrene spheres with sol-gel formulation and subsequent annealing. It possesses a relatively high degree of order among existing cell scaffolds and affords tight control over the scaffold porosity and tissue organization. The prepared scaffolds can be a convenient system for the investigation of cell-cell and cell-matrix interactions. Their biocompatibility is demonstrated for human hepatocellular carcinoma HEP G2 and human bone marrow HS-5 cell cultures. A preliminary effect of the scaffold topology on cell proliferation is observed. HEP G2 hepatocytes form a large number of 10-15 cell colonies on scaffolds made from 75-μm spheres, while their number diminishes for scaffolds from 10- and 160-μm spheres. Under similar conditions, HS-5 forms smaller colonies consisting of three to four cells in 90-μm cavities.
UR - http://www.scopus.com/inward/record.url?scp=4744371613&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4744371613&partnerID=8YFLogxK
U2 - 10.1021/la049958o
DO - 10.1021/la049958o
M3 - Article
C2 - 15350047
AN - SCOPUS:4744371613
VL - 20
SP - 7887
EP - 7892
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 19
ER -