Investigating and re-evaluating the role of glycogen synthase kinase 3 beta kinase as a molecular target for cardioprotection by using novel pharmacological inhibitors

Panagiota Efstathia Nikolaou, Kerstin Boengler, Panagiotis Efentakis, Konstantina Vouvogiannopoulou, Anastasia Zoga, Nicholas Gaboriaud-Kolar, Vassilios Myrianthopoulos, Pavlos Alexakos, Nikolaos Kostomitsopoulos, Ioannis Rerras, Anna Tsantili-Kakoulidou, Alexios Leandros Skaltsounis, Andreas Papapetropoulos, Efstathios K. Iliodromitis, Rainer Schulz, Ioanna Andreadou

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

AIMS: Glycogen synthase kinase 3 beta (GSK3β) link with the mitochondrial Permeability Transition Pore (mPTP) in cardioprotection is debated. We investigated the role of GSK3β in ischaemia (I)/reperfusion (R) injury using pharmacological tools. METHODS AND RESULTS: Infarct size using the GSK3β inhibitor BIO (6-bromoindirubin-3'-oxime) and several novel analogues (MLS2776-MLS2779) was determined in anaesthetized rabbits and mice. In myocardial tissue GSK3β inhibition and the specificity of the compounds was tested. The mechanism of protection focused on autophagy-related proteins. GSK3β localization was determined in subsarcolemmal (SSM) and interfibrillar mitochondria (IFM) isolated from Langendorff-perfused murine hearts (30'I/10'R or normoxic conditions). Calcium retention capacity (CRC) was determined in mitochondria after administration of the inhibitors in mice and in vitro. The effects of the inhibitors on mitochondrial respiration, reactive oxygen species (ROS) formation, ATP production, or hydrolysis were measured in SSM at baseline. Cyclosporine A (CsA) was co-administered with the inhibitors to address putative additive cardioprotective effects. Rabbits and mice treated with MLS compounds had smaller infarct size compared with control. In rabbits, MLS2776 and MLS2778 possessed greater infarct-sparing effects than BIO. GSK3β inhibition was confirmed at the 10th min and 2 h of reperfusion, while up-regulation of autophagy-related proteins was evident at late reperfusion. The mitochondrial amount of GSK3β was similar in normoxic SSM and IFM and was not altered by I/R. The inhibitors did not affect CRC or respiration, ROS and ATP production/hydrolysis at baseline. The co-administration of CsA ensured that cardioprotection was CypD-independent. CONCLUSION: Pharmacological inhibition of GSK3β attenuates infarct size beyond mPTP inhibition.

Original languageEnglish (US)
Pages (from-to)1228-1243
Number of pages16
JournalCardiovascular research
Volume115
Issue number7
DOIs
StatePublished - Jun 1 2019
Externally publishedYes

Fingerprint

Phosphotransferases
Pharmacology
Mitochondria
Rabbits
Cyclosporine
Reperfusion
Reactive Oxygen Species
Respiration
Hydrolysis
Adenosine Triphosphate
Calcium
Glycogen Synthase Kinase 3 beta
Reperfusion Injury
Up-Regulation
Ischemia

Keywords

  • Glycogen synthase kinase 3 beta (GSK3β)
  • Infarct size
  • Mitochondrial permeability transition pore
  • Novel selective analogues of BIO

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Investigating and re-evaluating the role of glycogen synthase kinase 3 beta kinase as a molecular target for cardioprotection by using novel pharmacological inhibitors. / Nikolaou, Panagiota Efstathia; Boengler, Kerstin; Efentakis, Panagiotis; Vouvogiannopoulou, Konstantina; Zoga, Anastasia; Gaboriaud-Kolar, Nicholas; Myrianthopoulos, Vassilios; Alexakos, Pavlos; Kostomitsopoulos, Nikolaos; Rerras, Ioannis; Tsantili-Kakoulidou, Anna; Skaltsounis, Alexios Leandros; Papapetropoulos, Andreas; Iliodromitis, Efstathios K.; Schulz, Rainer; Andreadou, Ioanna.

In: Cardiovascular research, Vol. 115, No. 7, 01.06.2019, p. 1228-1243.

Research output: Contribution to journalArticle

Nikolaou, PE, Boengler, K, Efentakis, P, Vouvogiannopoulou, K, Zoga, A, Gaboriaud-Kolar, N, Myrianthopoulos, V, Alexakos, P, Kostomitsopoulos, N, Rerras, I, Tsantili-Kakoulidou, A, Skaltsounis, AL, Papapetropoulos, A, Iliodromitis, EK, Schulz, R & Andreadou, I 2019, 'Investigating and re-evaluating the role of glycogen synthase kinase 3 beta kinase as a molecular target for cardioprotection by using novel pharmacological inhibitors', Cardiovascular research, vol. 115, no. 7, pp. 1228-1243. https://doi.org/10.1093/cvr/cvz061
Nikolaou, Panagiota Efstathia ; Boengler, Kerstin ; Efentakis, Panagiotis ; Vouvogiannopoulou, Konstantina ; Zoga, Anastasia ; Gaboriaud-Kolar, Nicholas ; Myrianthopoulos, Vassilios ; Alexakos, Pavlos ; Kostomitsopoulos, Nikolaos ; Rerras, Ioannis ; Tsantili-Kakoulidou, Anna ; Skaltsounis, Alexios Leandros ; Papapetropoulos, Andreas ; Iliodromitis, Efstathios K. ; Schulz, Rainer ; Andreadou, Ioanna. / Investigating and re-evaluating the role of glycogen synthase kinase 3 beta kinase as a molecular target for cardioprotection by using novel pharmacological inhibitors. In: Cardiovascular research. 2019 ; Vol. 115, No. 7. pp. 1228-1243.
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AU - Nikolaou, Panagiota Efstathia

AU - Boengler, Kerstin

AU - Efentakis, Panagiotis

AU - Vouvogiannopoulou, Konstantina

AU - Zoga, Anastasia

AU - Gaboriaud-Kolar, Nicholas

AU - Myrianthopoulos, Vassilios

AU - Alexakos, Pavlos

AU - Kostomitsopoulos, Nikolaos

AU - Rerras, Ioannis

AU - Tsantili-Kakoulidou, Anna

AU - Skaltsounis, Alexios Leandros

AU - Papapetropoulos, Andreas

AU - Iliodromitis, Efstathios K.

AU - Schulz, Rainer

AU - Andreadou, Ioanna

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