Investigation of the mechanism of the SpnF-catalyzed [4+2]-cycloaddition reaction in the biosynthesis of spinosyn A

Keyphrases

• Cyclization 100%
• Spinosyns 100%
• Cycloaddition Reaction 100%
• Kinetic Isotope Effect 100%
• Computational Results 50%
• Reaction Coordinate 50%
• Enzyme Active Site 50%
• Cyclase 50%
• Deuterium Isotope Effect 50%
• Insecticide 50%
• Enzymatic Reaction 50%
• Enzyme Catalysis 50%
• Cycloaddition 50%
• Catalytic Cycle 50%
• Secondary Metabolites 50%
• Non-enzymatic Reaction 50%
• Rehybridization 50%
• Conformational Restriction 50%
• Intermediary State 50%
• Six-membered Carbocycle 50%
• Cyclohexene 50%
• Saccharopolyspora Spinosa 50%
• Cyclization Mechanism 50%

Biochemistry, Genetics and Molecular Biology

• Anabolism 100%
• Enzyme 100%
• Kinetic Isotope Effect 100%
• Cycloaddition 100%
• Enzyme Active Site 33%
• Cyclase 33%
• Secondary Metabolite 33%
• Saccharopolyspora 33%

Chemistry

• Kinetic Isotope Effect 100%
• 1,4-cycloaddition 100%
• Spinosyn A 100%
• Chemistry 33%
• Metabolite 33%
• Enzymatic Reaction 33%
• Cyclohexene 33%
• formation 33%
• catalytic cycle 33%