Investigation of the subcellular localization of HPeV 3A and 2A proteins

Arsalan Salimi, Siamak Amirfakhri

Research output: Contribution to journalArticle

Abstract

Background: Our study investigated the sub-cellular localization of the Parechovirus HPeV 3A nonstructural protein. Methods: Primers were designed for making wild type HPeV1 and CAV9 2A, 2B, 2C, 2BC and 3A proteins to use for PCR and the pGEM-T Easy vector. The QuikChange II site-directed mutagenesis kit was used to mutate pEGFP-3A using primers. Cells were grown on cover slips and visualized using an Olympus BX41 fluorescence microscope. Results: We found that EGFP-3A co-localized partially with Golgi and ERGIC. The mutagenesis analysis suggests that the amino acids 39 to 86 of 3A affect distribution and are essential for localization. Conclusions: Interestingly, co-expression of HPeV 2A with HPeV 3A caused 2A translocation to the nucleolus. The deletion of the TM region in EGFP-2A prevents re-localization.

Original languageEnglish (US)
Pages (from-to)8573-8582
Number of pages10
JournalBiomedical Research (India)
Volume28
Issue number20
StatePublished - Jan 1 2017
Externally publishedYes

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Mutagenesis
Parechovirus
Site-Directed Mutagenesis
Proteins
Microscopes
Fluorescence
Amino Acids
Polymerase Chain Reaction

Keywords

  • HPeV 2A
  • HPeV 3A
  • Subcellular localization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Investigation of the subcellular localization of HPeV 3A and 2A proteins. / Salimi, Arsalan; Amirfakhri, Siamak.

In: Biomedical Research (India), Vol. 28, No. 20, 01.01.2017, p. 8573-8582.

Research output: Contribution to journalArticle

Salimi, A & Amirfakhri, S 2017, 'Investigation of the subcellular localization of HPeV 3A and 2A proteins', Biomedical Research (India), vol. 28, no. 20, pp. 8573-8582.
Salimi, Arsalan ; Amirfakhri, Siamak. / Investigation of the subcellular localization of HPeV 3A and 2A proteins. In: Biomedical Research (India). 2017 ; Vol. 28, No. 20. pp. 8573-8582.
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