Involvement of 3′,5′-cyclic inosine monophosphate in cystathionine γ-lyase-dependent regulation of the vascular tone

Emma Mitidieri, Valentina Vellecco, Vincenzo Brancaleone, Domenico Vanacore, Onorina L. Manzo, Emil Martin, Iraida Sharina, Yekaterina Krutsenko, Maria Chiara Monti, Elva Morretta, Andreas Papapetropoulos, Giuseppe Caliendo, Francesco Frecentese, Giuseppe Cirino, Raffaella Sorrentino, Roberta d'Emmanuele di Villa Bianca, Mariarosaria Bucci

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Background and Purpose: l-cysteine or hydrogen sulfide (H2S) donors induce a biphasic effect on precontracted isolated vessels. The contractile effect occurs within a concentration range of 10 nM to 3 μM followed by vasodilatation at 30–100 μM. Here, we have investigated the signalling involved in the H2S-induced contraction. Experimental Approach: Vascular response to NaHS or l-cysteine is evaluated on isolated precontracted with phenylephrine vessel rings harvested from wild type, cystathionine γ-lyase (CSE−/−), soluble guanylyl cyclase (sGCα1−/−) and endothelial nitric oxide synthase (eNOS−/−) knock-out mice. The cAMP, cGMP and inosine 3′,5′-cyclic monophosphate (cIMP) levels are simultaneously quantified using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis. The involvement of sGC, phosphodiesterase (PDE) 4A and PDE5 are also evaluated. Key Results: CSE-derived H2S-induced contraction requires an intact eNOS/NO/sGC pathway and involves cIMP as a second messenger. H2S contractile effect involves a transient increase of cGMP and cAMP metabolism caused by PDE5 and PDE4A, thus unmasking cIMP contracting action. The stable cell-permeable analogue of cIMP elicits concentration-dependent contraction on a stable background tone induced by phenylephrine. The lack of cIMP, coupled to the hypocontractility displayed by vessels harvested from CSE−/− mice, confirms that H2S-induced contraction involves cIMP. Conclusion and Implications: The endothelium dynamically regulates vessel homeostasis by modulating contractile tone. This also involves CSE-derived H2S that is mediated by cIMP.

Original languageEnglish (US)
Pages (from-to)3765-3782
Number of pages18
JournalBritish Journal of Pharmacology
Issue number18
StatePublished - Sep 2021


  • cyclic nucleotides
  • cystathionine γ-lyase
  • gasotransmitters
  • inosine 3′,5′-cyclic monophosphate
  • phosphodiesterases
  • vascular homeostasis
  • vasoconstriction

ASJC Scopus subject areas

  • Pharmacology


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