Involvement of calcitonin gene-related peptide in control of human fetoplacental vascular tone

Yuan Lin Dong, Sujatha Vegiraju, Madhu Chauhan, Pandu R R Gangula, Gary Hankins, Linda Goodrum, Chandra Yallampalli

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP), one of the most potent endogenous vasodilators known, has been implicated in vascular adaptations and placental functions during pregnancy. The present study was designed to examine the existence of CGRP-A receptor components, the calcitonin receptor-like receptor (CRLR) and receptor activity-modifying protein 1 (RAMP1), in the human placenta and the vasoactivity of CGRP in the fetoplacental circulation. Immunofluorescent staining of the human placenta in term labor using polyclonal anti-CRLR and RAMP1 antibodies revealed that labeling specifically concentrated in the vascular endothelium and the underlying smooth muscle cells in the umbilical artery/vein, chorionic artery/vein, and stem villous vessels as well as in the trophoblast layer of the placental villi. In vitro isometric force measurement showed that CGRP dose dependently relaxes the umbilical artery/vein, chorionic artery/vein, and stem villous vessels. Furthermore, CGRP-induced relaxation of placental vessels are inhibited by a CGRP receptor antagonist (CGRP8-37), ATP-sensitive potassium (KATP) channel blocker (glybenclamide), and cAMP-dependent protein kinase A inhibitor (Rp-cAMPS) and partially inhibited by a nitric oxide inhibitor (N ω-nitro-L-arginine methyl ester). We propose that CGRP may play a role in the control of human fetoplacental vascular tone, and the vascular dilations in response to CGRP may involve activation of K ATP channels, cAMP, and a nitric oxide pathway.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume286
Issue number1 55-1
StatePublished - Jan 2004

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Calcitonin Gene-Related Peptide
Blood Vessels
Receptor Activity-Modifying Protein 1
Calcitonin Receptor-Like Protein
Calcitonin Gene-Related Peptide Receptors
KATP Channels
Umbilical Veins
Umbilical Arteries
Cyclic AMP-Dependent Protein Kinases
Placenta
Veins
Nitric Oxide
Arteries
Potassium Channel Blockers
Placental Circulation
Chorionic Villi
Glyburide
Vascular Endothelium
Trophoblasts
Protein Kinase Inhibitors

Keywords

  • Cell signaling
  • Fetus
  • Pregnancy
  • Trophoblast
  • Vasoactivity

ASJC Scopus subject areas

  • Physiology

Cite this

Dong, Y. L., Vegiraju, S., Chauhan, M., Gangula, P. R. R., Hankins, G., Goodrum, L., & Yallampalli, C. (2004). Involvement of calcitonin gene-related peptide in control of human fetoplacental vascular tone. American Journal of Physiology - Heart and Circulatory Physiology, 286(1 55-1).

Involvement of calcitonin gene-related peptide in control of human fetoplacental vascular tone. / Dong, Yuan Lin; Vegiraju, Sujatha; Chauhan, Madhu; Gangula, Pandu R R; Hankins, Gary; Goodrum, Linda; Yallampalli, Chandra.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 286, No. 1 55-1, 01.2004.

Research output: Contribution to journalArticle

Dong, YL, Vegiraju, S, Chauhan, M, Gangula, PRR, Hankins, G, Goodrum, L & Yallampalli, C 2004, 'Involvement of calcitonin gene-related peptide in control of human fetoplacental vascular tone', American Journal of Physiology - Heart and Circulatory Physiology, vol. 286, no. 1 55-1.
Dong, Yuan Lin ; Vegiraju, Sujatha ; Chauhan, Madhu ; Gangula, Pandu R R ; Hankins, Gary ; Goodrum, Linda ; Yallampalli, Chandra. / Involvement of calcitonin gene-related peptide in control of human fetoplacental vascular tone. In: American Journal of Physiology - Heart and Circulatory Physiology. 2004 ; Vol. 286, No. 1 55-1.
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