Involvement of PKC, p38 MAPK and AP-2 in IL-1β-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells

Ping Chen, Ying Cai, Zhi Gang Yang, Rui Zhou, Guang Shen Zhang, Frederick Domann, Xiang Fang

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Objective: The aim of this study was to identify the signal molecules involved in IL-1β-induced expression of cyclooxygenase (COX)-2 in human pulmonary epithelial (A549) cells. Methods: A549 cells were stimulated with IL-1β in the presence or absence of H-7 (a protein kinase C inhibitor), SB203580 (a p38 mitogen-activated protein kinase inhibitor) and PD098059 (a mitogen-activated and extracellular regulated kinase kinase (MEK1) inhibitor). The A549 cells were also transfected with adenovirus vector encoding activator protein (AP)-2α, or a plasmid containing a dominant-negative gene (AP-2Δ), in the presence or absence of IL-1β. Results: IL-1β induced expression of the COX-2 mRNA and protein in A549 cells in a time- and dose-dependent manner. SB203580 and H-7, but not PD098059, inhibited IL-1β-induced expression of COX-2 protein. Overexpression of AP-2α increased expression of the COX-2 protein, whereas AP-2Δ decreased IL-1β-induced COX-2 expression. Conclusion: Protein kinase C, p38 mitogen-activated protein kinase and transcriptional factor AP-2α may play important roles in regulating IL-1β-induced COX-2 expression in human pulmonary epithelial cells.

Original languageEnglish (US)
Pages (from-to)18-23
Number of pages6
JournalRespirology
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

Keywords

  • Activator protein-2
  • Cyclooxygenase-2
  • Mitogen-activated and extracellular regulated kinase kinase 1
  • Mitogen-activated protein kinase
  • Protein kinase C

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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