Ion activation in electron capture dissociation to distinguish between N-terminal and C-terminal product ions

Yury O. Tsybin, Huan He, Mark Emmett, Christopher L. Hendrickson, Alan G. Marshall

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We present a method to distinguish N-terminal from C-terminal product ions in electron capture dissociation (ECD) MS/MS due to the change in relative abundances of even-electron (prime) and odd-electron (radical) product ions produced in consecutive ECD and activated ion-ECD mass spectra. The method is based on the rate and direction of hydrogen atom transfer between N-terminal and C-terminal ECD products and its dependence on ion internal energy. We demonstrate that increasing ion internal energy by vibrational activation prior to ECD results in decreased ratio of radical/prime N-terminal product ions (c./c′ ratio), but increased ratio of radical/prime C-terminal product ions (z./z′ ratio) in many cases. The combination of AI-ECD and ECD promises to increase the confidence of mass spectrometry-based peptide sequencing and protein identification.

Original languageEnglish (US)
Pages (from-to)7596-7602
Number of pages7
JournalAnalytical Chemistry
Volume79
Issue number20
DOIs
StatePublished - Oct 15 2007
Externally publishedYes

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Chemical activation
Ions
Electrons
Mass spectrometry
Hydrogen
Atoms
Peptides
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Ion activation in electron capture dissociation to distinguish between N-terminal and C-terminal product ions. / Tsybin, Yury O.; He, Huan; Emmett, Mark; Hendrickson, Christopher L.; Marshall, Alan G.

In: Analytical Chemistry, Vol. 79, No. 20, 15.10.2007, p. 7596-7602.

Research output: Contribution to journalArticle

Tsybin, Yury O. ; He, Huan ; Emmett, Mark ; Hendrickson, Christopher L. ; Marshall, Alan G. / Ion activation in electron capture dissociation to distinguish between N-terminal and C-terminal product ions. In: Analytical Chemistry. 2007 ; Vol. 79, No. 20. pp. 7596-7602.
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