Ion channels associated with the ectopic discharges generated after segmental spinal nerve injury in the rat

Xianzeng Liu, Jun Li Zhou, Kyungsoon Chung, Jin Chung

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

In an attempt to identify important ion channels contributing to the generation of ectopic discharges, the present study examined the effects of ion channel blockers on ectopic discharges of injured sensory neurons after spinal nerve ligation. The main focus of the study was to examine the effect of the sodium channel blocker, tetrodotoxin (TTX), in order to identify important subtype(s) (i.e. TTX-sensitive and TTX-resistant) of sodium channels that are involved in ectopic discharge generation. In addition, the effects of potassium and calcium channel blockers were also tested for comparison with the results of previous studies. The dorsal root ganglion (DRG) of the injured segment was removed along with the dorsal root (DR) and the spinal nerve 7-14 days after spinal nerve ligation in the rat. The tissue was placed in an in-vitro recording chamber consisting of multiple compartments that were independently perfused with 35°C artificial cerebrospinal fluid (ACSF). Single unit recordings were made from teased DR fibers. Once a spontaneously active unit was found and characterized, ACSF containing a channel blocker was perfused to the DRG, the site where almost all ectopic discharges originate after spinal nerve ligation. All the recorded spontaneously active units were found to be Aβ and Aδ fibers (no C fibers were detected). Perfusion of the DRG with a sodium channel blocker (lidocaine) at a dose much less than that required to block conduction of action potentials, significantly inhibited ectopic discharges in all recorded fibers. In addition, ectopic discharges were inhibited by TTX perfused to the DRG at a dose much lower (average of 22.1 nM) than that required to block TTX-resistant subtypes of sodium channels. The data suggest that TTX-sensitive sodium channels are likely to be involved in the generation of ectopic discharges. The present study also confirmed the results of previous studies on the additional potential roles of potassium and calcium channels, thus suggesting that multiple ion channels are likely to be involved in the generation of ectopic discharges.

Original languageEnglish (US)
Pages (from-to)119-127
Number of pages9
JournalBrain Research
Volume900
Issue number1
DOIs
StatePublished - May 4 2001

Fingerprint

Spinal Injuries
Spinal Nerves
Tetrodotoxin
Ion Channels
Spinal Ganglia
Sodium Channels
Sodium Channel Blockers
Ligation
Spinal Nerve Roots
Cerebrospinal Fluid
Potassium Channel Blockers
Myelinated Nerve Fibers
Unmyelinated Nerve Fibers
Potassium Channels
Calcium Channel Blockers
Sensory Receptor Cells
Calcium Channels
Lidocaine
Action Potentials
Perfusion

Keywords

  • Dorsal root ganglion (DRG)
  • Neuropathic pain
  • Sodium channel
  • Spinal nerve ligation model
  • Tetrodotoxin (TTX)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ion channels associated with the ectopic discharges generated after segmental spinal nerve injury in the rat. / Liu, Xianzeng; Zhou, Jun Li; Chung, Kyungsoon; Chung, Jin.

In: Brain Research, Vol. 900, No. 1, 04.05.2001, p. 119-127.

Research output: Contribution to journalArticle

Liu, Xianzeng ; Zhou, Jun Li ; Chung, Kyungsoon ; Chung, Jin. / Ion channels associated with the ectopic discharges generated after segmental spinal nerve injury in the rat. In: Brain Research. 2001 ; Vol. 900, No. 1. pp. 119-127.
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