Ionic channel currents in cultured neurons from human cortex

J. M. Simard, Y. Song, K. Tewari, S. Dunn, K. Werrbach-Perez, J. R. Perez-Polo, H. M. Eisenberg

    Research output: Contribution to journalArticle

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    Abstract

    Ionic channels in human cortical neurons have not been studied extensively. HCN-1 and HCN-1A cells, which recently were established as continuous cultures from human cortical tissue, have been shown by histochemical and immunochemical methods to exhibit a neuronal phenotype, but expression of functional ionic channels was not demonstrated. For the present study, HCN-1 and HCN-1A cells were cultured in Dulbecco's modified Eagle's medium with 15% fetal calf serum, in some cases supplemented with 10 ng/ml nerve growth factor, 10 μM forskolin, and 1 mM dibutyryl cyclic adenosine monophosphate to promote differentiation. Cells or membrane patches were voltage clamped using conventional patch clamp techniques. In HCN-1A cells, we identified a tetrodotoxin-sensitive Na+ current, two types of Ca2+ channel current, including L-type current and a second type that in some respects resembled N-type current, and four types of K+ current, including a delayed outward rectifier that showed voltage-dependent inactivation, two types of noninactivating Ca2+-activated K+ channels with slope conductances of 146 and 23 pS (K+(i)/K+(o) 145 mM/5 mM), and less frequently, a noninactivating, intermediate conductance channel that was not sensitive to internal Ca2+. When HCN-1A cells were examined after 3 days of exposure to differentiating agents, pronounced morphological changes were evident but no differences in ionic currents were apparent. HCN-1 cells also exhibited K+ and Ca2+ channel currents, but Na+ currents were not detected in these cells. Our data provide additional evidence indicating a functional neuronal phenotype for HCN-1A cells, and represent the most comprehensive survey to date of the variety of ionic channels expressed by human cortical neurons.

    Original languageEnglish (US)
    Pages (from-to)170-178
    Number of pages9
    JournalJournal of Neuroscience Research
    Volume34
    Issue number2
    DOIs
    StatePublished - 1993

    Fingerprint

    Ion Channels
    Neurons
    Phenotype
    Calcium-Activated Potassium Channels
    Eagles
    Tetrodotoxin
    Patch-Clamp Techniques
    Nerve Growth Factor
    Colforsin
    Cyclic AMP
    Cultured Cells
    Cell Membrane
    Serum

    Keywords

    • Ca channel
    • human
    • K channel
    • Na channel
    • neuron

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Simard, J. M., Song, Y., Tewari, K., Dunn, S., Werrbach-Perez, K., Perez-Polo, J. R., & Eisenberg, H. M. (1993). Ionic channel currents in cultured neurons from human cortex. Journal of Neuroscience Research, 34(2), 170-178. https://doi.org/10.1002/jnr.490340204

    Ionic channel currents in cultured neurons from human cortex. / Simard, J. M.; Song, Y.; Tewari, K.; Dunn, S.; Werrbach-Perez, K.; Perez-Polo, J. R.; Eisenberg, H. M.

    In: Journal of Neuroscience Research, Vol. 34, No. 2, 1993, p. 170-178.

    Research output: Contribution to journalArticle

    Simard, JM, Song, Y, Tewari, K, Dunn, S, Werrbach-Perez, K, Perez-Polo, JR & Eisenberg, HM 1993, 'Ionic channel currents in cultured neurons from human cortex', Journal of Neuroscience Research, vol. 34, no. 2, pp. 170-178. https://doi.org/10.1002/jnr.490340204
    Simard JM, Song Y, Tewari K, Dunn S, Werrbach-Perez K, Perez-Polo JR et al. Ionic channel currents in cultured neurons from human cortex. Journal of Neuroscience Research. 1993;34(2):170-178. https://doi.org/10.1002/jnr.490340204
    Simard, J. M. ; Song, Y. ; Tewari, K. ; Dunn, S. ; Werrbach-Perez, K. ; Perez-Polo, J. R. ; Eisenberg, H. M. / Ionic channel currents in cultured neurons from human cortex. In: Journal of Neuroscience Research. 1993 ; Vol. 34, No. 2. pp. 170-178.
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