IRES-based Venezuelan equine encephalitis vaccine candidate elicits protective immunity in mice

Shannan L. Rossi, Mathilde Guerbois, Rodion Gorchakov, Kenneth S. Plante, Naomi L. Forrester, Scott C. Weaver

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Venezuelan equine encephalitis virus (VEEV) is an arbovirus that causes periodic outbreaks that impact equine and human populations in the Americas. One of the VEEV subtypes located in Mexico and Central America (IE) has recently been recognized as an important cause of equine disease and death, and human exposure also appears to be widespread. Here, we describe the use of an Internal Ribosome Entry Site (IRES) from encephalomyocarditis virus to stably attenuate VEEV, creating a vaccine candidate independent of unstable point mutations. Mice infected with this virus produced antibodies and were protected against lethal VEEV challenge. This IRES-based vaccine was unable to establish productive infection in mosquito cell cultures or in intrathoracically injected Aedes taeniorhynchus, demonstrating that it cannot be transmitted from a vaccinee. These attenuation, efficacy and safety results justify further development for humans or equids of this new VEEV vaccine candidate.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalVirology
Volume437
Issue number2
DOIs
StatePublished - Mar 15 2013

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Keywords

  • Alphavirus
  • Internal ribosome entry site
  • Vaccine
  • Venezuelan equine encephalitis virus

ASJC Scopus subject areas

  • Virology

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