Iron(II)-ethylenediaminetetraacetic acid catalyzed cleavage of DNA is highly specific for duplex DNA

Maria J. Jezewska, Wlodzimierz Bujalowski, Timothy M. Lohman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We show that iron(II)-EDTA-catalyzed cleavage of duplex DNA is much more rapid than cleavage of single-stranded DNA that does not form intramolecular base pairs. Comparisons of the extent of cleavage of the fully single-stranded oligonucleotides d(pT)70, d(pA)70, and d(pC)35 and the duplex DNA d(pT)70·d(pA)70 indicate that the extent of cleavage increases significantly upon formation of the duplex structure. These observations indicate that accessibility of the DNA sugars to the presumed cleaving agent, hydroxyl radical, is not the major determinant for cleavage and that most likely a direct interaction between Fe(II) and the DNA is required. As a result, the interpretation of DNA cleavage experiments performed with this reagent to obtain detailed structural information should be pursued with caution until the mechanism of the cleavage reaction is better understood.

Original languageEnglish (US)
Pages (from-to)6161-6164
Number of pages4
JournalBiochemistry
Volume28
Issue number15
StatePublished - 1989
Externally publishedYes

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DNA Cleavage
Edetic Acid
Iron
DNA
Single-Stranded DNA
Oligonucleotides
Base Pairing
Hydroxyl Radical
Sugars
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Iron(II)-ethylenediaminetetraacetic acid catalyzed cleavage of DNA is highly specific for duplex DNA. / Jezewska, Maria J.; Bujalowski, Wlodzimierz; Lohman, Timothy M.

In: Biochemistry, Vol. 28, No. 15, 1989, p. 6161-6164.

Research output: Contribution to journalArticle

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