Is the CD18 adhesion complex of polymorphonuclear leukocytes involved in smoke-induced lung damage? A morphometric study

S. C. Guha, David Herndon, M. J. Evans, F. C. Schmalstieg, T. Isago, L. D. Traber, H. A. Linares, D. L. Traber

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Neutrophils are the primary vectors that produce lung parenchymal injury after smoke inhalation as a result of their transmigration through endothelial and epithelial gap junctions toward the airway surfaces. LFA-1 (CD11a/CD18) on the neutrophil surface is essential for this transmigration in many tissues. We hypothesized that anti-CD18 antibodies would block such movement and prevent lung damage. Ten sheep received nebulized cotton smoke in our standardized model of inhalation injury. The sham group (n = 2) was insufflated with air only, the second group (n = 4) was insufflated with smoke but received no antibody, and the third group (n = 4) was given monoclonal antibody R15.7 before smoke injury. A similar degree of parenchymal injury and an alveolar epithelial reparative response were measured in both groups receiving injury. Neutrophil counts in the interstitium of both injured groups were also similar. However, in the R15.7 pretreated group, neutrophils remained inside the capillary in far larger numbers (p < 0.05). The CD11/18 adhesion complex may not be necessary for the pulmonary microvascular changes associated with inhalation injury.

Original languageEnglish (US)
Pages (from-to)503-511
Number of pages9
JournalJournal of Burn Care and Rehabilitation
Volume14
Issue number5
DOIs
StatePublished - 1993

Fingerprint

Smoke
Neutrophils
Lung
Inhalation
Wounds and Injuries
Transendothelial and Transepithelial Migration
Lymphocyte Function-Associated Antigen-1
Gap Junctions
Lung Injury
Anti-Idiotypic Antibodies
Sheep
Air
Monoclonal Antibodies
Antibodies

ASJC Scopus subject areas

  • Rehabilitation
  • Surgery
  • Nursing(all)
  • Health Professions(all)
  • Emergency Medicine

Cite this

Is the CD18 adhesion complex of polymorphonuclear leukocytes involved in smoke-induced lung damage? A morphometric study. / Guha, S. C.; Herndon, David; Evans, M. J.; Schmalstieg, F. C.; Isago, T.; Traber, L. D.; Linares, H. A.; Traber, D. L.

In: Journal of Burn Care and Rehabilitation, Vol. 14, No. 5, 1993, p. 503-511.

Research output: Contribution to journalArticle

Guha, SC, Herndon, D, Evans, MJ, Schmalstieg, FC, Isago, T, Traber, LD, Linares, HA & Traber, DL 1993, 'Is the CD18 adhesion complex of polymorphonuclear leukocytes involved in smoke-induced lung damage? A morphometric study', Journal of Burn Care and Rehabilitation, vol. 14, no. 5, pp. 503-511. https://doi.org/10.1097/00004630-199309000-00003
Guha, S. C. ; Herndon, David ; Evans, M. J. ; Schmalstieg, F. C. ; Isago, T. ; Traber, L. D. ; Linares, H. A. ; Traber, D. L. / Is the CD18 adhesion complex of polymorphonuclear leukocytes involved in smoke-induced lung damage? A morphometric study. In: Journal of Burn Care and Rehabilitation. 1993 ; Vol. 14, No. 5. pp. 503-511.
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