Ischemic preconditioning in the isolated rat heart is not due to attenuation of free iron accumulation during prolonged ischemia

Gregory K. Asimakis

    Research output: Contribution to journalArticle

    Abstract

    Brief transient ischemia can protect the rat heart against a subsequent, prolonged period of ischemia (IS) . The cardio-protective mechanism of this phenomenon, known as ischémie preconditioning (IP), is unknown. Acid-stimulated release of bound iron during prolonged IS may produce free iron (FFe) which can catalyze the formation of potential1 y damaging hydroxyl radicals. Because IP is known to attenuate acidosis during prolonged IS, isolated rat hearts were used to test the following hypothesis: IP enhances the tolerance of the rat heart to T S by attenuating FFe accumulate on during 1S Control fC I hearts received 40 min of global ischemia 14 OIS) IF hearts were treated with 2 cycles of Smin ischemia/Smin reperfusion prior to 40IS. Myocardial pH was monitored with 3 mi c roe lee t rode inserted into the left, ventricular wall At the end of 4 OIS, the hearts were freeze - clamped, and the FFe contents of the homogenates were determined using a Fe- deferoxamine/HPLC assay Preischemic(Prel) FFe was determined in another group (n=6) which did not receive IS. After 401, myocardial pH was 5.96 £ O.ll and 6 39 ±0.12 for C (n = 6) and IP (n = 6) hearts, respectively (p < 0 05) Preischemic pH for both groups was approximately 7.2 FFe contents of Prel. C, and IP hearts were 13.6 ±1.0, 156 ±2.1; and 12.4 ±1.5 nmol /g. respectively {ANOVA, ns) . Therefore, IP is not due to attenuation of FFe accumulation. Also, myocardial injury resulting from prolonged IS does not appear to be due ischemia-induced accumulation of FFe in the rat heart rhi study was supported by NTH grant HL-50466.

    Original languageEnglish (US)
    JournalFASEB Journal
    Volume10
    Issue number3
    StatePublished - 1996

    Fingerprint

    Ischemic Preconditioning
    ischemia
    Rats
    Ischemia
    Iron
    heart
    iron
    rats
    deferoxamine
    Deferoxamine
    Organized Financing
    acidosis
    hydroxyl radicals
    Analysis of variance (ANOVA)
    Acidosis
    Hydroxyl Radical
    Reperfusion
    Assays
    Analysis of Variance
    analysis of variance

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences (miscellaneous)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Cell Biology

    Cite this

    Ischemic preconditioning in the isolated rat heart is not due to attenuation of free iron accumulation during prolonged ischemia. / Asimakis, Gregory K.

    In: FASEB Journal, Vol. 10, No. 3, 1996.

    Research output: Contribution to journalArticle

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