Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle

C. Eric Bailey, David W. Hammers, James H. DeFord, Vincent L. Dimayuga, James K. Amaning, Roger Farrar, John Papaconstantinou

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aging and skeletal muscle ischemia/reperfusion (I/R) injury leads to decreased contractile force generation that increases severely with age. Our studies show that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein expression is significantly decreased at 3 and 5 days reperfusion in the young mouse muscle and at 1, 3, 5, and 7 days in the aged muscle. Using PCR, we have shown that GAPDH mRNA levels in young and old muscle increase at 5 days reperfusion compared to control, suggesting that the protein deficit is not transcriptional. Furthermore, while total tyrosine nitration did not increase in the young muscle, GAPDH nitration increased significantly at 1 and 3 days reperfusion. In contrast, total tyrosine nitration in aged muscle increased significantly at 1, 3, and 5 days of reperfusion, with increases in GAPDH nitration at the same time points. We conclude that GAPDH protein levels decrease following I/R, that this is not transcriptionally mediated, that the aged muscle experiences greater oxidative stress, protein modification and GAPDH degradation, possibly contributing to decreased muscle function. We propose that tyrosine nitration enhances GAPDH degradation following I/R and that the persistent decrease of GAPDH in aged muscle is due to the prolonged increase in oxidative modification in this age group.

Original languageEnglish (US)
Pages (from-to)1003-1017
Number of pages15
JournalAging
Volume3
Issue number10
StatePublished - Oct 2011

Fingerprint

Glyceraldehyde-3-Phosphate Dehydrogenases
Reperfusion
Skeletal Muscle
Muscles
Tyrosine
Ischemia
Proteins
Heat-Shock Proteins
Reperfusion Injury
Oxidative Stress
Age Groups
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Aging
  • Gapdh
  • Ischemia/reperfusion
  • Nitration
  • Skeletal muscle

ASJC Scopus subject areas

  • Aging
  • Cell Biology

Cite this

Bailey, C. E., Hammers, D. W., DeFord, J. H., Dimayuga, V. L., Amaning, J. K., Farrar, R., & Papaconstantinou, J. (2011). Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle. Aging, 3(10), 1003-1017.

Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle. / Bailey, C. Eric; Hammers, David W.; DeFord, James H.; Dimayuga, Vincent L.; Amaning, James K.; Farrar, Roger; Papaconstantinou, John.

In: Aging, Vol. 3, No. 10, 10.2011, p. 1003-1017.

Research output: Contribution to journalArticle

Bailey, CE, Hammers, DW, DeFord, JH, Dimayuga, VL, Amaning, JK, Farrar, R & Papaconstantinou, J 2011, 'Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle', Aging, vol. 3, no. 10, pp. 1003-1017.
Bailey CE, Hammers DW, DeFord JH, Dimayuga VL, Amaning JK, Farrar R et al. Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle. Aging. 2011 Oct;3(10):1003-1017.
Bailey, C. Eric ; Hammers, David W. ; DeFord, James H. ; Dimayuga, Vincent L. ; Amaning, James K. ; Farrar, Roger ; Papaconstantinou, John. / Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle. In: Aging. 2011 ; Vol. 3, No. 10. pp. 1003-1017.
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