TY - JOUR
T1 - Isoelectric focusing by free solutioncapillary electrophoresis
AU - Chen, Shiaw Min
AU - Wiktorowicz, John E.
PY - 1992/10
Y1 - 1992/10
N2 - A reproducible, quantitative isoelectric focusing method using capillary electrophoresis that exhibits high resolution and linearity over a wide pH gradient was developed. RNase T1 and RNase ba are two proteins that have isoelectric points (pI's) at the two extremes of a pH 3-10 gradient. Site-directed mutants of the former were separated from the wild-type form and pI's determined in the same experiment. The pI's of RNase T1 wild-type, its three mutants, and RNase ba were determined for the first time as 2.9, 3.1, 3.1, 3.3, and 9.0, respectively. The paper describes the protocol for isoelectric focusing by capillary electrophoresis, as well as presenting data describing the linearity, resolution, limits of mass loading, and reproducibility of the method.
AB - A reproducible, quantitative isoelectric focusing method using capillary electrophoresis that exhibits high resolution and linearity over a wide pH gradient was developed. RNase T1 and RNase ba are two proteins that have isoelectric points (pI's) at the two extremes of a pH 3-10 gradient. Site-directed mutants of the former were separated from the wild-type form and pI's determined in the same experiment. The pI's of RNase T1 wild-type, its three mutants, and RNase ba were determined for the first time as 2.9, 3.1, 3.1, 3.3, and 9.0, respectively. The paper describes the protocol for isoelectric focusing by capillary electrophoresis, as well as presenting data describing the linearity, resolution, limits of mass loading, and reproducibility of the method.
UR - http://www.scopus.com/inward/record.url?scp=0026795391&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026795391&partnerID=8YFLogxK
U2 - 10.1016/S0003-2697(05)80014-4
DO - 10.1016/S0003-2697(05)80014-4
M3 - Article
C2 - 1456446
AN - SCOPUS:0026795391
SN - 0003-2697
VL - 206
SP - 84
EP - 90
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -