Isolation and characterization of a new antitumor polysaccharide, KS-2, extracted from culture mycelia of Lentinus edodes

T. Fujii, H. Maeda, Fujio Suzuki, N. Ishida

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

A new antitumor and antiviral substance, KS-2, was prepared by ethanol precipitation of the hot water extract of culture mycelia of Lentinus edodes KSLE 007. It was further purified by ECTEOLA-cellulose and Sephadex G-100 column chromatography based on the interferon-inducing activity. Its homogeneity was revealed by CsCl density gradient centrifugation, electrophoresis on cellulose acetate and Sephadex G-100 and ECTEOLA-cellulose column chromatography. KS-2 is mainly composed of α-linked mannose and contains a small amount of peptide which consists of serine, threonine and alanine with residual amounts of the other amino acids. The estimated molecular weight of KS-2 is between 6.0 x 104 and 9.5 x 104. KS-2 suppressed the growth of Ehrlich as well as Sarcoma-180 tumors in mice when given either orally or intraperitoneally. It is also capable of inducing an interferon in mice when dosed orally or intraperitoneally. The acute LD50 of KS-2 was found to be extremely low, more than 12,500 mg/kg when administered orally to mice.

Original languageEnglish (US)
Pages (from-to)1079-1090
Number of pages12
JournalJournal of Antibiotics
Volume31
Issue number11
StatePublished - 1978
Externally publishedYes

Fingerprint

Shiitake Mushrooms
Mycelium
Polysaccharides
Interferons
Chromatography
Cellulose Acetate Electrophoresis
Sarcoma 180
Density Gradient Centrifugation
Lethal Dose 50
Threonine
Mannose
Alanine
Serine
Antiviral Agents
Ethanol
Molecular Weight
krestin
Amino Acids
Peptides
Water

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Isolation and characterization of a new antitumor polysaccharide, KS-2, extracted from culture mycelia of Lentinus edodes. / Fujii, T.; Maeda, H.; Suzuki, Fujio; Ishida, N.

In: Journal of Antibiotics, Vol. 31, No. 11, 1978, p. 1079-1090.

Research output: Contribution to journalArticle

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