TY - JOUR
T1 - Isolation and characterization of a new antitumor polysaccharide, KS-2, extracted from culture mycelia of lentinus edodes
AU - Fujii, Toshikatsu
AU - Maeda, Hiroshi
AU - Suzuki, Fujio
AU - Ishida, Nakao
PY - 1978/1/1
Y1 - 1978/1/1
N2 - A new antitumor and antiviral substance, KS-2, was prepared by ethanol precipitation of the hot water extract of culture mycelia of Lentinus edodes KSLE 007. It was further purified by ECTEOLA-cellulose and Sephadex G-100 column chromatography based on the interferon-inducing activity. Its homogeneity was revealed by CsCl density gradient centrifugation, electrophoresis on cellulose acetate and Sephadex G-100 and ECTEOLA-cellulose column chromatography. KS-2 is mainly composed of a-linked mannose and contains a small amount of peptide which consists of serine, threonine and alanine with residual amounts of the other amino acids. The estimated molecular weight of KS-2 is between 6.0×104 and 9.5×104. KS-2 suppressed the growth of Ehrlich as well as Sarcoma-180 tumors in mice when given either orally or intraperitoneally. It is also capable of inducing an interferon in mice when dosed orally or intraperitoneally. The acute LD50 of KS-2 was found to be extremely low, more than 12,500mg/kg when administered orally to mice.
AB - A new antitumor and antiviral substance, KS-2, was prepared by ethanol precipitation of the hot water extract of culture mycelia of Lentinus edodes KSLE 007. It was further purified by ECTEOLA-cellulose and Sephadex G-100 column chromatography based on the interferon-inducing activity. Its homogeneity was revealed by CsCl density gradient centrifugation, electrophoresis on cellulose acetate and Sephadex G-100 and ECTEOLA-cellulose column chromatography. KS-2 is mainly composed of a-linked mannose and contains a small amount of peptide which consists of serine, threonine and alanine with residual amounts of the other amino acids. The estimated molecular weight of KS-2 is between 6.0×104 and 9.5×104. KS-2 suppressed the growth of Ehrlich as well as Sarcoma-180 tumors in mice when given either orally or intraperitoneally. It is also capable of inducing an interferon in mice when dosed orally or intraperitoneally. The acute LD50 of KS-2 was found to be extremely low, more than 12,500mg/kg when administered orally to mice.
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U2 - 10.7164/antibiotics.31.1079
DO - 10.7164/antibiotics.31.1079
M3 - Article
C2 - 569140
AN - SCOPUS:0018232617
VL - 31
SP - 1079
EP - 1090
JO - Journal of Antibiotics
JF - Journal of Antibiotics
SN - 0021-8820
IS - 11
ER -