Abstract
Lytic coliphage vB_EcoP_G7C and several other highly related isolates were obtained repeatedly from the samples of horse feces held in the same stable thus representing a component of the normal indigenous intestinal communities in this population of animals. The genome of G7C consists of 71,759. bp with terminal repeats of about 1160. bp, yielding approximately 73 kbp packed DNA size. Seventy-eight potential open reading frames, most of them unique to N4-like viruses, were identified and annotated. The overall layout of functional gene groups was close to that of the original N4 phage, with some important changes in late gene area including new tail fiber proteins containing hydrolytic domains. Structural proteome analysis confirmed all the predicted subunits of the viral particle. Unlike N4 itself, phage G7C did not exhibit a lysis-inhibited phenotype.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 93-99 |
| Number of pages | 7 |
| Journal | Virology |
| Volume | 426 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 10 2012 |
| Externally published | Yes |
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Keywords
- Complete genome sequence
- Enterobacteria bacteriophage
- Escherichia coli
- Lytic phage
- N4
- N4-like coliphage
- Podoviridae
- Proteomics
- Sequence analysis
- Tail fiber
ASJC Scopus subject areas
- Virology
Cite this
Isolation and characterization of a novel indigenous intestinal N4-related coliphage vB_EcoP_G7C. / Kulikov, Eugene; Kropinski, Andrew M.; Golomidova, Alla; Lingohr, Erika; Govorun, Vadim; Serebryakova, Marina; Prokhorov, Nikolai; Letarova, Maria; Manykin, Anatolij; Strotskaya, Alexandra; Letarov, Andrey.
In: Virology, Vol. 426, No. 2, 10.05.2012, p. 93-99.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of a novel indigenous intestinal N4-related coliphage vB_EcoP_G7C
AU - Kulikov, Eugene
AU - Kropinski, Andrew M.
AU - Golomidova, Alla
AU - Lingohr, Erika
AU - Govorun, Vadim
AU - Serebryakova, Marina
AU - Prokhorov, Nikolai
AU - Letarova, Maria
AU - Manykin, Anatolij
AU - Strotskaya, Alexandra
AU - Letarov, Andrey
PY - 2012/5/10
Y1 - 2012/5/10
N2 - Lytic coliphage vB_EcoP_G7C and several other highly related isolates were obtained repeatedly from the samples of horse feces held in the same stable thus representing a component of the normal indigenous intestinal communities in this population of animals. The genome of G7C consists of 71,759. bp with terminal repeats of about 1160. bp, yielding approximately 73 kbp packed DNA size. Seventy-eight potential open reading frames, most of them unique to N4-like viruses, were identified and annotated. The overall layout of functional gene groups was close to that of the original N4 phage, with some important changes in late gene area including new tail fiber proteins containing hydrolytic domains. Structural proteome analysis confirmed all the predicted subunits of the viral particle. Unlike N4 itself, phage G7C did not exhibit a lysis-inhibited phenotype.
AB - Lytic coliphage vB_EcoP_G7C and several other highly related isolates were obtained repeatedly from the samples of horse feces held in the same stable thus representing a component of the normal indigenous intestinal communities in this population of animals. The genome of G7C consists of 71,759. bp with terminal repeats of about 1160. bp, yielding approximately 73 kbp packed DNA size. Seventy-eight potential open reading frames, most of them unique to N4-like viruses, were identified and annotated. The overall layout of functional gene groups was close to that of the original N4 phage, with some important changes in late gene area including new tail fiber proteins containing hydrolytic domains. Structural proteome analysis confirmed all the predicted subunits of the viral particle. Unlike N4 itself, phage G7C did not exhibit a lysis-inhibited phenotype.
KW - Complete genome sequence
KW - Enterobacteria bacteriophage
KW - Escherichia coli
KW - Lytic phage
KW - N4
KW - N4-like coliphage
KW - Podoviridae
KW - Proteomics
KW - Sequence analysis
KW - Tail fiber
UR - http://www.scopus.com/inward/record.url?scp=84857784353&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84857784353&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2012.01.027
DO - 10.1016/j.virol.2012.01.027
M3 - Article
C2 - 22341309
AN - SCOPUS:84857784353
VL - 426
SP - 93
EP - 99
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -