Isolation and Characterization of cDNAs That Encode Homologs of a Pathogenesis-related Protein Allergen from Cryptomeri

Norihiro Futamura; Yuzuru Mukai; Masahiro Sakaguchi; Hiroshi Yasueda; Sakae Inouye; Terumi Midoro-Horiuti; Randall M. Goldblum; Kenji Shinohara

doi:10.1271/bbb.66.2495

Isolation and Characterization of cDNAs That Encode Homologs of a Pathogenesis-related Protein Allergen from Cryptomeri

Norihiro Futamura, Yuzuru Mukai, Masahiro Sakaguchi, Hiroshi Yasueda, Sakae Inouye, Terumi Midoro-Horiuti, Randall M. Goldblum, Kenji Shinohara

Pediatrics

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Many plant pathogenesis-related (PR) proteins are allergenic. We isolated three cDNAs, Cry j 3.1, Cry j 3.2, and Cry j 3.3, that encoded homologs of Jun a 3, a PR protein allergen in Juniperus ashei, from a cDNA library derived from the pollen of Cryptomeria japonica. The predicted amino acid sequences encoded by the three cDNAs were more than 85% identical to each other and about 57% identical to the sequence of Jun a 3. The Cry j 3 genes seemed to form a small multigene family in the genome of C. japonica. Expression of Cry j 3 was strong in roots and in female and male strobili; expression was weaker in cotyledons, leaves, stems, and pollen grains.

Original language	English (US)
Pages (from-to)	2495-2500
Number of pages	6
Journal	Bioscience, Biotechnology and Biochemistry
Volume	66
Issue number	11
DOIs	https://doi.org/10.1271/bbb.66.2495
State	Published - Jan 1 2002

Keywords

Cdna cloning
Cryptomeria japonica
Pathogenesis-related protein
Pollen allergen

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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@article{051f377686e34ac0ab264db818aef31a,

title = "Isolation and Characterization of cDNAs That Encode Homologs of a Pathogenesis-related Protein Allergen from Cryptomeri",

abstract = "Many plant pathogenesis-related (PR) proteins are allergenic. We isolated three cDNAs, Cry j 3.1, Cry j 3.2, and Cry j 3.3, that encoded homologs of Jun a 3, a PR protein allergen in Juniperus ashei, from a cDNA library derived from the pollen of Cryptomeria japonica. The predicted amino acid sequences encoded by the three cDNAs were more than 85% identical to each other and about 57% identical to the sequence of Jun a 3. The Cry j 3 genes seemed to form a small multigene family in the genome of C. japonica. Expression of Cry j 3 was strong in roots and in female and male strobili; expression was weaker in cotyledons, leaves, stems, and pollen grains.",

keywords = "Cdna cloning, Cryptomeria japonica, Pathogenesis-related protein, Pollen allergen",

author = "Norihiro Futamura and Yuzuru Mukai and Masahiro Sakaguchi and Hiroshi Yasueda and Sakae Inouye and Terumi Midoro-Horiuti and Goldblum, {Randall M.} and Kenji Shinohara",

year = "2002",

month = jan,

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doi = "10.1271/bbb.66.2495",

language = "English (US)",

volume = "66",

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T1 - Isolation and Characterization of cDNAs That Encode Homologs of a Pathogenesis-related Protein Allergen from Cryptomeri

AU - Futamura, Norihiro

AU - Mukai, Yuzuru

AU - Sakaguchi, Masahiro

AU - Yasueda, Hiroshi

AU - Inouye, Sakae

AU - Midoro-Horiuti, Terumi

AU - Goldblum, Randall M.

AU - Shinohara, Kenji

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Many plant pathogenesis-related (PR) proteins are allergenic. We isolated three cDNAs, Cry j 3.1, Cry j 3.2, and Cry j 3.3, that encoded homologs of Jun a 3, a PR protein allergen in Juniperus ashei, from a cDNA library derived from the pollen of Cryptomeria japonica. The predicted amino acid sequences encoded by the three cDNAs were more than 85% identical to each other and about 57% identical to the sequence of Jun a 3. The Cry j 3 genes seemed to form a small multigene family in the genome of C. japonica. Expression of Cry j 3 was strong in roots and in female and male strobili; expression was weaker in cotyledons, leaves, stems, and pollen grains.

AB - Many plant pathogenesis-related (PR) proteins are allergenic. We isolated three cDNAs, Cry j 3.1, Cry j 3.2, and Cry j 3.3, that encoded homologs of Jun a 3, a PR protein allergen in Juniperus ashei, from a cDNA library derived from the pollen of Cryptomeria japonica. The predicted amino acid sequences encoded by the three cDNAs were more than 85% identical to each other and about 57% identical to the sequence of Jun a 3. The Cry j 3 genes seemed to form a small multigene family in the genome of C. japonica. Expression of Cry j 3 was strong in roots and in female and male strobili; expression was weaker in cotyledons, leaves, stems, and pollen grains.

KW - Cdna cloning

KW - Cryptomeria japonica

KW - Pathogenesis-related protein

KW - Pollen allergen

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AN - SCOPUS:0344180968

SN - 0916-8451

VL - 66

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EP - 2500

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 11

ER -

Isolation and Characterization of cDNAs That Encode Homologs of a Pathogenesis-related Protein Allergen from Cryptomeri

Abstract

Keywords

ASJC Scopus subject areas

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