Isolation and characterization of cDNAs that encode homologs of a pathogenesis-related protein allergen from Cryptomeria japonica

Norihiro Futamura, Yuzuru Mukai, Masahiro Sakaguchi, Hiroshi Yasueda, Sakae Inouye, Terumi Midoro-Horiuti, Randall M. Goldblum, Kenji Shinohara

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Many plant pathogenesis-related (PR) proteins are allergenic. We isolated three cDNAs, Cry j 3.1, Cry j 3.2, and Cry j 3.3, that encoded homologs of Jun a 3, a PR protein allergen in Juniperus ashei, from a cDNA library derived from the pollen of Cryptomeria japonica. The predicted amino acid sequences encoded by the three cDNAs were more than 85% identical to each other and about 57% identical to the sequence of Jun a 3. The Cry j 3 genes seemed to form a small multigene family in the genome of C. japonica. Expression of Cry j 3 was strong in roots and in female and male strobili; expression was weaker in cotyledons, leaves, stems, and pollen grains.

Original languageEnglish (US)
Pages (from-to)2495-2500
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number11
StatePublished - Nov 2002

Fingerprint

Cryptomeria
Allergens
Cryptomeria japonica
pathogenesis-related proteins
Pollen
allergens
Juniperus ashei
Complementary DNA
Genes
Juniperus
pollen
Proteins
Cotyledon
Multigene Family
plant proteins
Gene Library
multigene family
cDNA libraries
cotyledons
Amino acids

Keywords

  • cDNA cloning
  • Cryptomeria japonica
  • Pathogenesis-related protein
  • Pollen allergen

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Isolation and characterization of cDNAs that encode homologs of a pathogenesis-related protein allergen from Cryptomeria japonica. / Futamura, Norihiro; Mukai, Yuzuru; Sakaguchi, Masahiro; Yasueda, Hiroshi; Inouye, Sakae; Midoro-Horiuti, Terumi; Goldblum, Randall M.; Shinohara, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 66, No. 11, 11.2002, p. 2495-2500.

Research output: Contribution to journalArticle

Futamura, N, Mukai, Y, Sakaguchi, M, Yasueda, H, Inouye, S, Midoro-Horiuti, T, Goldblum, RM & Shinohara, K 2002, 'Isolation and characterization of cDNAs that encode homologs of a pathogenesis-related protein allergen from Cryptomeria japonica', Bioscience, Biotechnology and Biochemistry, vol. 66, no. 11, pp. 2495-2500.
Futamura, Norihiro ; Mukai, Yuzuru ; Sakaguchi, Masahiro ; Yasueda, Hiroshi ; Inouye, Sakae ; Midoro-Horiuti, Terumi ; Goldblum, Randall M. ; Shinohara, Kenji. / Isolation and characterization of cDNAs that encode homologs of a pathogenesis-related protein allergen from Cryptomeria japonica. In: Bioscience, Biotechnology and Biochemistry. 2002 ; Vol. 66, No. 11. pp. 2495-2500.
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