Isolation and Characterization of cDNAs That Encode Homologs of a Pathogenesis-related Protein Allergen from Cryptomeri

Norihiro Futamura, Yuzuru Mukai, Masahiro Sakaguchi, Hiroshi Yasueda, Sakae Inouye, Terumi Midoro-Horiuti, Randall M. Goldblum, Kenji Shinohara

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Many plant pathogenesis-related (PR) proteins are allergenic. We isolated three cDNAs, Cry j 3.1, Cry j 3.2, and Cry j 3.3, that encoded homologs of Jun a 3, a PR protein allergen in Juniperus ashei, from a cDNA library derived from the pollen of Cryptomeria japonica. The predicted amino acid sequences encoded by the three cDNAs were more than 85% identical to each other and about 57% identical to the sequence of Jun a 3. The Cry j 3 genes seemed to form a small multigene family in the genome of C. japonica. Expression of Cry j 3 was strong in roots and in female and male strobili; expression was weaker in cotyledons, leaves, stems, and pollen grains.

Original languageEnglish (US)
Pages (from-to)2495-2500
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number11
DOIs
StatePublished - Jan 1 2002

Keywords

  • Cdna cloning
  • Cryptomeria japonica
  • Pathogenesis-related protein
  • Pollen allergen

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Isolation and Characterization of cDNAs That Encode Homologs of a Pathogenesis-related Protein Allergen from Cryptomeri'. Together they form a unique fingerprint.

  • Cite this