Isolation and characterization of genomic clones for two chicken phenobarbital-inducible cytochrome P-450 genes

Lisa Elferink, A. A. Hobbs, A. W. Steggles, B. K. May, W. H. Elliott

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A cDNA clone specific for a chicken phenobarbital-inducible cytochrome P-450 was used to screen a chicken genomic library. Twenty-nine clones were isolated, restriction mapped, and divided into two nonoverlapping groups. The cDNA clone hybridized to 12 kilobases of DNA from both groups. Both groups contained restriction fragments which hybridized to both 5' and 3' fragments of the cDNA clone, and it was concluded that the two groups were derived from two separate genes. Southern transfer analysis of individual chicken DNAs and quantitative hybridization analysis indicated that these two genes are independent and are present as single copies/haploid genome. Comparison of restriction digests of the cloned DNAs and total genomic DNA discounted the possibility that other closely related P-450 genes are present in the chicken genome.

Original languageEnglish (US)
Pages (from-to)9438-9443
Number of pages6
JournalJournal of Biological Chemistry
Volume261
Issue number20
StatePublished - 1986
Externally publishedYes

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Phenobarbital
Cytochrome P-450 Enzyme System
Chickens
Clone Cells
Genes
Complementary DNA
DNA
Genome
Genomic Library
Haploidy
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation and characterization of genomic clones for two chicken phenobarbital-inducible cytochrome P-450 genes. / Elferink, Lisa; Hobbs, A. A.; Steggles, A. W.; May, B. K.; Elliott, W. H.

In: Journal of Biological Chemistry, Vol. 261, No. 20, 1986, p. 9438-9443.

Research output: Contribution to journalArticle

Elferink, Lisa ; Hobbs, A. A. ; Steggles, A. W. ; May, B. K. ; Elliott, W. H. / Isolation and characterization of genomic clones for two chicken phenobarbital-inducible cytochrome P-450 genes. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 20. pp. 9438-9443.
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