Isolation and characterization of human amniotic fluid-derived exosomes

Samantha Sheller-Miller, Ramkumar Menon

    Research output: Chapter in Book/Report/Conference proceedingChapter

    8 Scopus citations

    Abstract

    Extracellular vesicles (EVs), specifically exosomes of 50–150 nm, have emerged as important communication channels between cells and tissues and can be isolated from multiple biofluids including blood, urine and amniotic fluid. No standardized approach for exosome isolation from these biofluids has been established. This chapter outlines an optimized approach for isolating exosomes from human amniotic fluid samples. Like plasma, amniotic fluid contains many protein and cellular contaminants that requires multiple steps for cleanup. Therefore, to ensure samples contain minimal contaminants, including larger EVs, we also outline multiple methods for characterization of isolated exosomes for size, morphology and protein markers.

    Original languageEnglish (US)
    Title of host publicationExtracellular vesicles
    EditorsSheila Spada, Lorenzo Galluzzi
    PublisherAcademic Press Inc.
    Pages181-194
    Number of pages14
    ISBN (Print)9780128206621
    DOIs
    StatePublished - Jan 2020

    Publication series

    NameMethods in Enzymology
    Volume645
    ISSN (Print)0076-6879
    ISSN (Electronic)1557-7988

    Keywords

    • Extracellular vesicles
    • Parturition
    • Preterm labor
    • Purification
    • Term labor

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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