Isolation and characterization of human amniotic fluid-derived exosomes

Samantha Sheller-Miller, Ramkumar Menon

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Extracellular vesicles (EVs), specifically exosomes of 50–150 nm, have emerged as important communication channels between cells and tissues and can be isolated from multiple biofluids including blood, urine and amniotic fluid. No standardized approach for exosome isolation from these biofluids has been established. This chapter outlines an optimized approach for isolating exosomes from human amniotic fluid samples. Like plasma, amniotic fluid contains many protein and cellular contaminants that requires multiple steps for cleanup. Therefore, to ensure samples contain minimal contaminants, including larger EVs, we also outline multiple methods for characterization of isolated exosomes for size, morphology and protein markers.

Original languageEnglish (US)
Title of host publicationExtracellular vesicles
EditorsSheila Spada, Lorenzo Galluzzi
PublisherAcademic Press Inc.
Pages181-194
Number of pages14
ISBN (Print)9780128206621
DOIs
StatePublished - Jan 2020
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume645
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Extracellular vesicles
  • Parturition
  • Preterm labor
  • Purification
  • Term labor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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