Isolation and characterization of native Cry j 3 from Japanese cedar (Cryptomeria japonica) pollen

T. Fujimura, N. Futamura, Terumi Midoro-Horiuti, A. Togawa, R. M. Goldblum, H. Yasueda, A. Saito, K. Shinohara, K. Masuda, K. Kurata, M. Sakaguchi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Japanese cedar (Cryptomeria japonica) pollinosis is the most prevalent allergy in Japan. Recently, the Japanese cedar pollen allergen Cry j 3 was cloned as a homologue of Jun a 3, which is a major allergen from mountain cedar (Juniperus ashei) pollen. However, native Cry j 3 has not been isolated and there are no reports on its allergenic activity. The aims of this study were to isolate native Cry j 3 and assess its immunoglobulin E (IgE)-binding capacity in patients with Japanese cedar pollinosis. Methods: Native Cry j 3 was purified from Japanese cedar pollen by multidimensional chromatography. We assessed the IgE-binding capacity using sera from patients allergic to Japanese cedar pollen by immunoblot analysis and ELISA. Moreover, we assayed the capacity of Cry j 3 to induce histamine release from the patients' leukocytes. We cloned cDNA corresponding to purified Cry j 3 from a cDNA library of Japanese cedar pollen. Results: We isolated native Cry j 3 as a 27-kDa protein. The IgE-binding frequency of Cry j 3 from the sera of patients allergic to Japanese cedar pollen was estimated as 27% (27/100) by ELISA. Cry j 3 induced the release of histamine from leukocytes. We cloned the cDNA and named it Cry j 3.8. Cry j 3.8 cDNA encoded 225 amino acids and had significant homology with thaumatin-like proteins. Conclusions: Cry j 3 is a causative allergen in Japanese cedar pollinosis and may play crucial roles in the cross-reactivity with oral allergy syndrome.

Original languageEnglish (US)
Pages (from-to)547-553
Number of pages7
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume62
Issue number5
DOIs
StatePublished - May 2007

Fingerprint

Cryptomeria
Pollen
Seasonal Allergic Rhinitis
Allergens
Immunoglobulin E
Complementary DNA
Histamine Release
Hypersensitivity
Leukocytes
Enzyme-Linked Immunosorbent Assay
Juniperus
Serum
Gene Library
Chromatography
Japan
Proteins
Amino Acids

Keywords

  • Japanese cedar
  • Oral allergy syndrome
  • Pathogenesis-related protein
  • Pollinosis
  • Thaumatin-like protein

ASJC Scopus subject areas

  • Immunology

Cite this

Isolation and characterization of native Cry j 3 from Japanese cedar (Cryptomeria japonica) pollen. / Fujimura, T.; Futamura, N.; Midoro-Horiuti, Terumi; Togawa, A.; Goldblum, R. M.; Yasueda, H.; Saito, A.; Shinohara, K.; Masuda, K.; Kurata, K.; Sakaguchi, M.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 62, No. 5, 05.2007, p. 547-553.

Research output: Contribution to journalArticle

Fujimura, T, Futamura, N, Midoro-Horiuti, T, Togawa, A, Goldblum, RM, Yasueda, H, Saito, A, Shinohara, K, Masuda, K, Kurata, K & Sakaguchi, M 2007, 'Isolation and characterization of native Cry j 3 from Japanese cedar (Cryptomeria japonica) pollen', Allergy: European Journal of Allergy and Clinical Immunology, vol. 62, no. 5, pp. 547-553. https://doi.org/10.1111/j.1398-9995.2007.01331.x
Fujimura, T. ; Futamura, N. ; Midoro-Horiuti, Terumi ; Togawa, A. ; Goldblum, R. M. ; Yasueda, H. ; Saito, A. ; Shinohara, K. ; Masuda, K. ; Kurata, K. ; Sakaguchi, M. / Isolation and characterization of native Cry j 3 from Japanese cedar (Cryptomeria japonica) pollen. In: Allergy: European Journal of Allergy and Clinical Immunology. 2007 ; Vol. 62, No. 5. pp. 547-553.
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abstract = "Background: Japanese cedar (Cryptomeria japonica) pollinosis is the most prevalent allergy in Japan. Recently, the Japanese cedar pollen allergen Cry j 3 was cloned as a homologue of Jun a 3, which is a major allergen from mountain cedar (Juniperus ashei) pollen. However, native Cry j 3 has not been isolated and there are no reports on its allergenic activity. The aims of this study were to isolate native Cry j 3 and assess its immunoglobulin E (IgE)-binding capacity in patients with Japanese cedar pollinosis. Methods: Native Cry j 3 was purified from Japanese cedar pollen by multidimensional chromatography. We assessed the IgE-binding capacity using sera from patients allergic to Japanese cedar pollen by immunoblot analysis and ELISA. Moreover, we assayed the capacity of Cry j 3 to induce histamine release from the patients' leukocytes. We cloned cDNA corresponding to purified Cry j 3 from a cDNA library of Japanese cedar pollen. Results: We isolated native Cry j 3 as a 27-kDa protein. The IgE-binding frequency of Cry j 3 from the sera of patients allergic to Japanese cedar pollen was estimated as 27{\%} (27/100) by ELISA. Cry j 3 induced the release of histamine from leukocytes. We cloned the cDNA and named it Cry j 3.8. Cry j 3.8 cDNA encoded 225 amino acids and had significant homology with thaumatin-like proteins. Conclusions: Cry j 3 is a causative allergen in Japanese cedar pollinosis and may play crucial roles in the cross-reactivity with oral allergy syndrome.",
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AU - Fujimura, T.

AU - Futamura, N.

AU - Midoro-Horiuti, Terumi

AU - Togawa, A.

AU - Goldblum, R. M.

AU - Yasueda, H.

AU - Saito, A.

AU - Shinohara, K.

AU - Masuda, K.

AU - Kurata, K.

AU - Sakaguchi, M.

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N2 - Background: Japanese cedar (Cryptomeria japonica) pollinosis is the most prevalent allergy in Japan. Recently, the Japanese cedar pollen allergen Cry j 3 was cloned as a homologue of Jun a 3, which is a major allergen from mountain cedar (Juniperus ashei) pollen. However, native Cry j 3 has not been isolated and there are no reports on its allergenic activity. The aims of this study were to isolate native Cry j 3 and assess its immunoglobulin E (IgE)-binding capacity in patients with Japanese cedar pollinosis. Methods: Native Cry j 3 was purified from Japanese cedar pollen by multidimensional chromatography. We assessed the IgE-binding capacity using sera from patients allergic to Japanese cedar pollen by immunoblot analysis and ELISA. Moreover, we assayed the capacity of Cry j 3 to induce histamine release from the patients' leukocytes. We cloned cDNA corresponding to purified Cry j 3 from a cDNA library of Japanese cedar pollen. Results: We isolated native Cry j 3 as a 27-kDa protein. The IgE-binding frequency of Cry j 3 from the sera of patients allergic to Japanese cedar pollen was estimated as 27% (27/100) by ELISA. Cry j 3 induced the release of histamine from leukocytes. We cloned the cDNA and named it Cry j 3.8. Cry j 3.8 cDNA encoded 225 amino acids and had significant homology with thaumatin-like proteins. Conclusions: Cry j 3 is a causative allergen in Japanese cedar pollinosis and may play crucial roles in the cross-reactivity with oral allergy syndrome.

AB - Background: Japanese cedar (Cryptomeria japonica) pollinosis is the most prevalent allergy in Japan. Recently, the Japanese cedar pollen allergen Cry j 3 was cloned as a homologue of Jun a 3, which is a major allergen from mountain cedar (Juniperus ashei) pollen. However, native Cry j 3 has not been isolated and there are no reports on its allergenic activity. The aims of this study were to isolate native Cry j 3 and assess its immunoglobulin E (IgE)-binding capacity in patients with Japanese cedar pollinosis. Methods: Native Cry j 3 was purified from Japanese cedar pollen by multidimensional chromatography. We assessed the IgE-binding capacity using sera from patients allergic to Japanese cedar pollen by immunoblot analysis and ELISA. Moreover, we assayed the capacity of Cry j 3 to induce histamine release from the patients' leukocytes. We cloned cDNA corresponding to purified Cry j 3 from a cDNA library of Japanese cedar pollen. Results: We isolated native Cry j 3 as a 27-kDa protein. The IgE-binding frequency of Cry j 3 from the sera of patients allergic to Japanese cedar pollen was estimated as 27% (27/100) by ELISA. Cry j 3 induced the release of histamine from leukocytes. We cloned the cDNA and named it Cry j 3.8. Cry j 3.8 cDNA encoded 225 amino acids and had significant homology with thaumatin-like proteins. Conclusions: Cry j 3 is a causative allergen in Japanese cedar pollinosis and may play crucial roles in the cross-reactivity with oral allergy syndrome.

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