Isolation and characterization of ovine gamma globulins

Ole Aalund, John W. Osebold, Frederick A. Murphy

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Fractions of sheep serum were obtained by anion-exchange column chromatography on DEAE Sephadex, and gel filtration on Sephadex G-200 which contained only one of the following ovine gamma globulins: 7S gamma-2, 7S gamma-1, and gamma-1M. The gamma globulins were identified by immuno-electrophoresis, starch-gel electrophoresis, analytical ultracentrifugation, and by their content of antibody activity. By these physical criteria they were identical to their counterparts in human serum. Circumstantial evidence indicated that ovine serum also contains a protein analogous to the gamma-1A globulin of human serum.

Original languageEnglish (US)
Pages (from-to)142-149
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume109
Issue number1
StatePublished - Jan 1965

Fingerprint

gamma-Globulins
Sheep
Immunoglobulin G
DEAE-Dextran
Column chromatography
Globulins
Electrophoresis
Serum
Starch
Anions
Ion exchange
Gels
Starch Gel Electrophoresis
Antibodies
Ultracentrifugation
Gel Chromatography
Chromatography
Proteins
sephadex
Immunoelectrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Aalund, O., Osebold, J. W., & Murphy, F. A. (1965). Isolation and characterization of ovine gamma globulins. Archives of Biochemistry and Biophysics, 109(1), 142-149.

Isolation and characterization of ovine gamma globulins. / Aalund, Ole; Osebold, John W.; Murphy, Frederick A.

In: Archives of Biochemistry and Biophysics, Vol. 109, No. 1, 01.1965, p. 142-149.

Research output: Contribution to journalArticle

Aalund, O, Osebold, JW & Murphy, FA 1965, 'Isolation and characterization of ovine gamma globulins', Archives of Biochemistry and Biophysics, vol. 109, no. 1, pp. 142-149.
Aalund, Ole ; Osebold, John W. ; Murphy, Frederick A. / Isolation and characterization of ovine gamma globulins. In: Archives of Biochemistry and Biophysics. 1965 ; Vol. 109, No. 1. pp. 142-149.
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