Isolation and characterization of ovine gamma globulins

Ole Aalund; John W. Osebold; Frederick A. Murphy

doi:10.1016/0003-9861(65)90299-7

Isolation and characterization of ovine gamma globulins

Ole Aalund
, John W. Osebold
, Frederick A. Murphy

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Fractions of sheep serum were obtained by anion-exchange column chromatography on DEAE Sephadex, and gel filtration on Sephadex G-200 which contained only one of the following ovine gamma globulins: 7S gamma-2, 7S gamma-1, and gamma-1M. The gamma globulins were identified by immuno-electrophoresis, starch-gel electrophoresis, analytical ultracentrifugation, and by their content of antibody activity. By these physical criteria they were identical to their counterparts in human serum. Circumstantial evidence indicated that ovine serum also contains a protein analogous to the gamma-1A globulin of human serum.

Original language	English (US)
Pages (from-to)	142-149
Number of pages	8
Journal	Archives of Biochemistry and Biophysics
Volume	109
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(65)90299-7
State	Published - Jan 1965
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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title = "Isolation and characterization of ovine gamma globulins",

abstract = "Fractions of sheep serum were obtained by anion-exchange column chromatography on DEAE Sephadex, and gel filtration on Sephadex G-200 which contained only one of the following ovine gamma globulins: 7S gamma-2, 7S gamma-1, and gamma-1M. The gamma globulins were identified by immuno-electrophoresis, starch-gel electrophoresis, analytical ultracentrifugation, and by their content of antibody activity. By these physical criteria they were identical to their counterparts in human serum. Circumstantial evidence indicated that ovine serum also contains a protein analogous to the gamma-1A globulin of human serum.",

author = "Ole Aalund and Osebold, \{John W.\} and Murphy, \{Frederick A.\}",

note = "Funding Information: 1 These studies were supported in part, b> research grant A-1080 from the National Institutes of Health, IT. S. Public Health Service. 2 Present address : Hygienic-Ractrriological Laboratory of the Royal 1{\textquoteright}cterinary and Agricul- tural College, Bidowsvej 13, Copenhagen T-. Denmark.",

year = "1965",

month = jan,

doi = "10.1016/0003-9861(65)90299-7",

language = "English (US)",

volume = "109",

pages = "142--149",

journal = "Archives of Biochemistry and Biophysics",

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T1 - Isolation and characterization of ovine gamma globulins

AU - Aalund, Ole

AU - Osebold, John W.

AU - Murphy, Frederick A.

N1 - Funding Information: 1 These studies were supported in part, b> research grant A-1080 from the National Institutes of Health, IT. S. Public Health Service. 2 Present address : Hygienic-Ractrriological Laboratory of the Royal 1’cterinary and Agricul- tural College, Bidowsvej 13, Copenhagen T-. Denmark.

PY - 1965/1

Y1 - 1965/1

N2 - Fractions of sheep serum were obtained by anion-exchange column chromatography on DEAE Sephadex, and gel filtration on Sephadex G-200 which contained only one of the following ovine gamma globulins: 7S gamma-2, 7S gamma-1, and gamma-1M. The gamma globulins were identified by immuno-electrophoresis, starch-gel electrophoresis, analytical ultracentrifugation, and by their content of antibody activity. By these physical criteria they were identical to their counterparts in human serum. Circumstantial evidence indicated that ovine serum also contains a protein analogous to the gamma-1A globulin of human serum.

AB - Fractions of sheep serum were obtained by anion-exchange column chromatography on DEAE Sephadex, and gel filtration on Sephadex G-200 which contained only one of the following ovine gamma globulins: 7S gamma-2, 7S gamma-1, and gamma-1M. The gamma globulins were identified by immuno-electrophoresis, starch-gel electrophoresis, analytical ultracentrifugation, and by their content of antibody activity. By these physical criteria they were identical to their counterparts in human serum. Circumstantial evidence indicated that ovine serum also contains a protein analogous to the gamma-1A globulin of human serum.

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U2 - 10.1016/0003-9861(65)90299-7

DO - 10.1016/0003-9861(65)90299-7

M3 - Article

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EP - 149

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

IS - 1

ER -

Isolation and characterization of ovine gamma globulins

Abstract

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